Structure-oriented substrate specificity engineering of aldehyde-deformylating oxygenase towards aldehydes carbon chain length

Table 4 Fatty alk(a/e)ne production in genetically engineered E. coli strains

mg/L/OD	ΔfadE (BL21)	LB99	LB100	LB101
n-Undecane	ND	ND	0.044 ± 0.0030	0.078 ± 0.0010
n-Pentadecane	ND	ND	0.066 ± 0.011	0.016 ± 0.0028
8-Heptadecene	ND	ND	0.30 ± 0.023	ND
n-Dodecanol	ND	0.72 ± 0.0035	ND	ND
n-Dodecanoic acid	0.16 ± 0.014	1.1 ± 0.12	0.41 ± 0.039	0.50 ± 0.060
n-Tetradecanoic acid	1.43 ± 0.050	1.0 ± 0.015	1.0 ± 0.16	0.95 ± 0.055
n-Hexadecanoic acid	15.1 ± 2.1	1.8 ± 0.030	2.8 ± 0.32	4.1 ± 0.27
n-Octadecanoic acid	14.9 ± 1.6	0.62 ± 0.025	1.1 ± 0.12	0.67 ± 0.077

Yields of fatty alk(a/e)nes and others produced by genetically engineered E. coli strains were quantified by GC–MS
ND not determined due to low concentrations

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