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Table 4 Fatty alk(a/e)ne production in genetically engineered E. coli strains

From: Structure-oriented substrate specificity engineering of aldehyde-deformylating oxygenase towards aldehydes carbon chain length

mg/L/OD ΔfadE (BL21) LB99 LB100 LB101
n-Undecane ND ND 0.044 ± 0.0030 0.078 ± 0.0010
n-Pentadecane ND ND 0.066 ± 0.011 0.016 ± 0.0028
8-Heptadecene ND ND 0.30 ± 0.023 ND
n-Dodecanol ND 0.72 ± 0.0035 ND ND
n-Dodecanoic acid 0.16 ± 0.014 1.1 ± 0.12 0.41 ± 0.039 0.50 ± 0.060
n-Tetradecanoic acid 1.43 ± 0.050 1.0 ± 0.015 1.0 ± 0.16 0.95 ± 0.055
n-Hexadecanoic acid 15.1 ± 2.1 1.8 ± 0.030 2.8 ± 0.32 4.1 ± 0.27
n-Octadecanoic acid 14.9 ± 1.6 0.62 ± 0.025 1.1 ± 0.12 0.67 ± 0.077
  1. Yields of fatty alk(a/e)nes and others produced by genetically engineered E. coli strains were quantified by GC–MS
  2. ND not determined due to low concentrations