Fig. 1

Schematic view of PCR-based gene disruption (a) and the mutant confirmation by PCR (b). a The PUT cassette obtained via PCR using Up-F/Down-R was used to generate the sdh5::URA3 disruption by homologous recombination. Clones that contain URA₃ can grow on the selected plate. b Diagnostic PCR using extracted genomic DNA of the transformants as template with two sets of primer pairs of chrom-F/ura-R (left) and chrom-F/chrom-R (right). M 1 kb DNA marker ladder (MBI). 1, 2, 3 were three independent transformants. Transformant 3 was verified to be positive