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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: From mannan to bioethanol: cell surface co-display of β-mannanase and β-mannosidase on yeast Saccharomyces cerevisiae

Fig. 6

Repeated ethanol fermentation from 1,4-β-d-mannan using Man5A- and Mnd2A-displaying yeast cells. a Mock/Mock yeast cells. b Man5A-Disp/Mnd2A-Disp yeast cells. Fermentations were performed in SCA media (pH 5.0) containing 5 g/L 1,4-β-d-mannan as a carbon source. First batch fermentations were started with an OD600 = 0.05 of yeast cells. For second and third batch fermentations, the cells were collected, washed, and resuspended in fresh SCA media containing 5 g/L 1,4-β-d-mannan. Data are presented as the mean ± standard deviation of separate cultivations (n = 3 each)

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