Plasmid | Specific features | Source |
---|---|---|
pGK426 | Yeast multi-copy expression vector containing PGK1 promoter, PGK1 terminator, 2μ origin, and URA3 marker | [28] |
pFGK426 | Yeast cell surface-display vector using Flo428 anchor protein; contains coding sequences for secretion signal sequence of α-factor and Flo428 anchor (C-terminal 428 a.a. of Flo1p) in pGK426 | [26] |
pFGK426-AaMan5A | A. aculeatus β-mannanase (Man5A) display in pFGK426; fused with FLAG tag at the C-terminus | This study |
pFGK426-AaMan5A-TAA | A. aculeatus β-mannanase (Man5A) secretion in pFGK426; fused with FLAG tag at the C-terminus | This study |
pGK424 | Yeast multi-copy expression vector containing PGK1 promoter, PGK1 terminator, 2μ origin, and TRP1 marker | [28] |
pFGK424 | Yeast cell surface-display vector using Flo428 anchor protein; contains coding sequences for secretion signal sequence of α-factor and Flo428 anchor in pGK424 | This study |
pFGK424-AaMnd2A | A. aculeatus β-mannosidase (Mnd2A) display in pFGK424; fused with HA tag at the C-terminus | This study |
pFGK424-AaMnd2A-TAA | A. aculeatus β-mannosidase (Mnd2A) secretion in pFGK424; fused with HA tag at the C-terminus | This study |
pRS406 | Yeast integration vector containing URA3 marker | ATCC |
pFGK406-AaMan5A | Yeast integration plasmid for displaying A. aculeatus β-mannanase (Man5A) using pFGK426 expression cassette | This study |
pRS402 | Yeast integration vector containing ADE2 marker | ATCC |
pFGK402-AaMnd2A | Yeast integration plasmid for displaying A. aculeatus β-mannosidase (Mnd2A) using pFGK424 expression cassette | This study |