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Table 1 Plasmids used in this study

From: From mannan to bioethanol: cell surface co-display of β-mannanase and β-mannosidase on yeast Saccharomyces cerevisiae

Plasmid

Specific features

Source

pGK426

Yeast multi-copy expression vector containing PGK1 promoter, PGK1 terminator, 2μ origin, and URA3 marker

[28]

pFGK426

Yeast cell surface-display vector using Flo428 anchor protein; contains coding sequences for secretion signal sequence of α-factor and Flo428 anchor (C-terminal 428 a.a. of Flo1p) in pGK426

[26]

pFGK426-AaMan5A

A. aculeatus β-mannanase (Man5A) display in pFGK426; fused with FLAG tag at the C-terminus

This study

pFGK426-AaMan5A-TAA

A. aculeatus β-mannanase (Man5A) secretion in pFGK426; fused with FLAG tag at the C-terminus

This study

pGK424

Yeast multi-copy expression vector containing PGK1 promoter, PGK1 terminator, 2μ origin, and TRP1 marker

[28]

pFGK424

Yeast cell surface-display vector using Flo428 anchor protein; contains coding sequences for secretion signal sequence of α-factor and Flo428 anchor in pGK424

This study

pFGK424-AaMnd2A

A. aculeatus β-mannosidase (Mnd2A) display in pFGK424; fused with HA tag at the C-terminus

This study

pFGK424-AaMnd2A-TAA

A. aculeatus β-mannosidase (Mnd2A) secretion in pFGK424; fused with HA tag at the C-terminus

This study

pRS406

Yeast integration vector containing URA3 marker

ATCC

pFGK406-AaMan5A

Yeast integration plasmid for displaying A. aculeatus β-mannanase (Man5A) using pFGK426 expression cassette

This study

pRS402

Yeast integration vector containing ADE2 marker

ATCC

pFGK402-AaMnd2A

Yeast integration plasmid for displaying A. aculeatus β-mannosidase (Mnd2A) using pFGK424 expression cassette

This study