Table 1 Comparison of hydrogenase activity in wt (CC-124 and D66) and Fd-HydA transformants (1D4 and P6)

Photosynthetic H₂ production [µmol(H₂) mg(chl)⁻¹ h⁻¹]

8.34 ± 1.4

21.65 ± 1.5

0.91 ± 0.027

6.06 ± 0.538

Chemical H₂ production [µmol(H₂) mg(chl)⁻¹ h⁻¹]

37.68 ± 8

104.6 ± 14.4

4.5 ± 0.55

26.9 ± 0.298

Amount of enzyme [ng(enzyme) mg(chl)⁻¹]

628

1743

21

128

Amount of enzyme [µmol(enzyme) mg(chl)⁻¹]

1.31 × 10⁻⁵

3.63 × 10⁻⁵

0.35 × 10⁻⁶

2.14 × 10⁻⁶

Photosynthetic H₂ production rate standardized for hydrogenase amount (PHPRH) [µmol(H₂) µmol(enzyme)⁻¹ min⁻¹]

10,622

9931

42,909

47,236

Ratio of PHPRH (transformant/wt average)

~1

~1

4.2

4.6