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Table 1 Comparison of hydrogenase activity in wt (CC-124 and D66) and Fd-HydA transformants (1D4 and P6)

From: The dual effect of a ferredoxin-hydrogenase fusion protein in vivo: successful divergence of the photosynthetic electron flux towards hydrogen production and elevated oxygen tolerance

  D66 (HydA) CC-124 (HydA) 1D4 (Fd-HydA) P6 (Fd-HydA)
Photosynthetic H2 production [µmol(H2) mg(chl)−1 h−1] 8.34 ± 1.4 21.65 ± 1.5 0.91 ± 0.027 6.06 ± 0.538
Chemical H2 production [µmol(H2) mg(chl)−1 h−1] 37.68 ± 8 104.6 ± 14.4 4.5 ± 0.55 26.9 ± 0.298
Amount of enzyme [ng(enzyme) mg(chl)−1] 628 1743 21 128
Amount of enzyme [µmol(enzyme) mg(chl)−1] 1.31 × 10−5 3.63 × 10−5 0.35 × 10−6 2.14 × 10−6
Photosynthetic H2 production rate standardized for hydrogenase amount (PHPRH) [µmol(H2) µmol(enzyme)−1 min−1] 10,622 9931 42,909 47,236
Ratio of PHPRH (transformant/wt average) ~1 ~1 4.2 4.6
  1. All of the experiments were performed in biological triplicates