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Table 1 The source and components of the 2,3-BDO pathway constructed and the corresponding 2,3-BDO titer (g/L) after 24-h post-inoculation in Z. mobilis 8b

From: Metabolic engineering of Zymomonas mobilis for 2,3-butanediol production from lignocellulosic biomass sugars

Strain or plasmid Characteristic(s) Source or reference
Plasmids
 pEZ15Asp (pEZ) Shuttle vector contains Z. mobilis origin and E. coli origin p15A; SpR; Biobrick-compatible This work
 pEZ-GS6 pEZ containing construct GS6: aldC and bdh genes from E. cloacae driven by its native promoter [PEc_(ALDC-BDH)] This work
 pEZ-GS2 pEZ containing construct GS2: codon-optimized aldC and bdh genes from E. cloacae driven by Pgap promoter [GS2: Pgap_(ALDC-BDH) This work
 pEZ-BC3 pEZ containing construct BC3: codon-optimized aldC and bdh genes from B. licheniformis driven by Pgap and Peno promoter respectively [(Pgap_ALDC)-(Peno_BDH)] This work
 pEZ-BC4 pEZ containing construct BC4: alS gene from E. cloacae driven by its native promoter (PEc_ALSecwt) and GS6 construct [(PEc_ALSecwt)-GS6] This work
 pEZ-BC5 pEZ containing construct BC5: codon-optimized alS gene from E. cloacae driven by Peno (Peno_ALSecopt) and GS2 construct [(Peno_ALSecopt)-GS2] This work
 pEZ-BC9 pEZ containing construct BC9: codon-optimized alS gene from B. licheniformis driven by inducible promoter Ptet (Ptet_BlAls) and GS2 construct [(Ptet-BlAls)-GS2] This work
 pEZ-BC10 pEZ containing construct BC10: codon-optimized alS gene from B. subtilis driven by inducible promoter Ptet (Ptet-BsAls1) and GS2 construct [(Ptet-BsAls1)-GS2] This work
 pEZ-BC11 pEZ containing construct BC11: 2nd version of codon-optimized alS gene from B. subtilis driven by inducible promoter Ptet (Ptet-BsAlss) and GS2 construct [(Ptet-BsAls2)-GS2] This work
Bacterial strains
 E. coli C2925 ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) TcS endA1 rspL136 (StrR) dam13::Tn9 (CmR) xylA-5 mtl-1 thi-1 mcrB1 hsdR2 NEB
 8b Engineered Z. mobilis subsp. ZM4 strain for xylose utilization, TcRCmR [37]
 9C 8b derivative with tetracycline and chloramphenicol antibiotics markers cured, TcSCmS Lab stock
 22C 8b derivate with enhanced corn stover hydrolysate tolerance, TcRCmR [44]
 8b-pEZ Z. mobilis 8b containing control plasmid pEZ This work
 8b-GS2 Z. mobilis 8b containing plasmid pEZ-GS2 This work
 8b-GS6 Z. mobilis 8b containing plasmid pEZ-GS6 This work
 8b-BC3 Z. mobilis 8b containing plasmid pEZ-BC3 This work
 8b-BC4 Z. mobilis 8b containing plasmid pEZ-BC4 This work
 8b-BC5 Z. mobilis 8b containing plasmid pEZ-BC5 This work
 22C-pEZ Z. mobilis 22C containing control plasmid pEZ This work
 22C-BC5 Z. mobilis 22C containing plasmid pEZ-BC5 This work
 9C-BC5 Z. mobilis 9C containing plasmid pEZ-BC5 This work
 9C-BC9 Z. mobilis 9C containing plasmid pEZ-BC9 This work
 9C-BC10 Z. mobilis 9C containing plasmid pEZ-BC10 This work
 9C-BC11 Z. mobilis 9C containing plasmid pEZ-BC11 This work
  1. Culture condition: 40 mL RMG8 in 125-mL flask, 33 °C, 120 rpm
  2. EC E. cloacea; BL B. lincheniformis. Sp R spectinomycin resistance; Tc R tetracycline resistance; Cm R chloramphenicol resistance; Tc S tetracycline sensitive; Cm R chloramphenicol sensitive