Table 1 The source and components of the 2,3-BDO pathway constructed and the corresponding 2,3-BDO titer (g/L) after 24-h post-inoculation in Z. mobilis 8b
Strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
Plasmids | ||
 pEZ15Asp (pEZ) | Shuttle vector contains Z. mobilis origin and E. coli origin p15A; SpR; Biobrick-compatible | This work |
 pEZ-GS6 | pEZ containing construct GS6: aldC and bdh genes from E. cloacae driven by its native promoter [PEc_(ALDC-BDH)] | This work |
 pEZ-GS2 | pEZ containing construct GS2: codon-optimized aldC and bdh genes from E. cloacae driven by Pgap promoter [GS2: Pgap_(ALDC-BDH) | This work |
 pEZ-BC3 | pEZ containing construct BC3: codon-optimized aldC and bdh genes from B. licheniformis driven by Pgap and Peno promoter respectively [(Pgap_ALDC)-(Peno_BDH)] | This work |
 pEZ-BC4 | pEZ containing construct BC4: alS gene from E. cloacae driven by its native promoter (PEc_ALSecwt) and GS6 construct [(PEc_ALSecwt)-GS6] | This work |
 pEZ-BC5 | pEZ containing construct BC5: codon-optimized alS gene from E. cloacae driven by Peno (Peno_ALSecopt) and GS2 construct [(Peno_ALSecopt)-GS2] | This work |
 pEZ-BC9 | pEZ containing construct BC9: codon-optimized alS gene from B. licheniformis driven by inducible promoter Ptet (Ptet_BlAls) and GS2 construct [(Ptet-BlAls)-GS2] | This work |
 pEZ-BC10 | pEZ containing construct BC10: codon-optimized alS gene from B. subtilis driven by inducible promoter Ptet (Ptet-BsAls1) and GS2 construct [(Ptet-BsAls1)-GS2] | This work |
 pEZ-BC11 | pEZ containing construct BC11: 2nd version of codon-optimized alS gene from B. subtilis driven by inducible promoter Ptet (Ptet-BsAlss) and GS2 construct [(Ptet-BsAls2)-GS2] | This work |
Bacterial strains | ||
 E. coli C2925 | ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 R(zgb210::Tn10) TcS endA1 rspL136 (StrR) dam13::Tn9 (CmR) xylA-5 mtl-1 thi-1 mcrB1 hsdR2 | NEB |
 8b | Engineered Z. mobilis subsp. ZM4 strain for xylose utilization, TcRCmR | [37] |
 9C | 8b derivative with tetracycline and chloramphenicol antibiotics markers cured, TcSCmS | Lab stock |
 22C | 8b derivate with enhanced corn stover hydrolysate tolerance, TcRCmR | [44] |
 8b-pEZ | Z. mobilis 8b containing control plasmid pEZ | This work |
 8b-GS2 | Z. mobilis 8b containing plasmid pEZ-GS2 | This work |
 8b-GS6 | Z. mobilis 8b containing plasmid pEZ-GS6 | This work |
 8b-BC3 | Z. mobilis 8b containing plasmid pEZ-BC3 | This work |
 8b-BC4 | Z. mobilis 8b containing plasmid pEZ-BC4 | This work |
 8b-BC5 | Z. mobilis 8b containing plasmid pEZ-BC5 | This work |
 22C-pEZ | Z. mobilis 22C containing control plasmid pEZ | This work |
 22C-BC5 | Z. mobilis 22C containing plasmid pEZ-BC5 | This work |
 9C-BC5 | Z. mobilis 9C containing plasmid pEZ-BC5 | This work |
 9C-BC9 | Z. mobilis 9C containing plasmid pEZ-BC9 | This work |
 9C-BC10 | Z. mobilis 9C containing plasmid pEZ-BC10 | This work |
 9C-BC11 | Z. mobilis 9C containing plasmid pEZ-BC11 | This work |