Table 2 The source and components of the 2,3-BDO pathway constructed and the corresponding 2,3-BDO titer (g/L) after 24-h post-inoculation in Z. mobilis 8b
Strain | Plasmid | Promoter | Species | Enzymes | Codon optimization | BDO (g/L) |
|---|---|---|---|---|---|---|
8b-GS6 | pEZ-GS6 | Wild-type | E. cloacea | AldC, Bdh | No | 1.42 |
8b-GS2 | pEZ-GS2 | Pgap | E. cloacea | AldC, Bdh | Yes | 3.69 |
8b-BC3 | pEZ-BC3 | Pgap, Peno | B. lincheniformis | AldC, Bdh | Yes | 2.88 |
8b-BC4 | pEZ-BC4 | Wild-type | E. cloacea | Als, AldC, Bdh | No | 1.9 |
8b-BC5 | pEZ-BC5 | Peno, Pgap | E. cloacea | Als, AldC, Bdh | Yes | 5.0 |