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Table 3 The source and components of the 2,3-BDO pathway constructed and the corresponding BDO titer 1 day post-inoculation in Z. mobilis 9C

From: Metabolic engineering of Zymomonas mobilis for 2,3-butanediol production from lignocellulosic biomass sugars

Strain Plasmid Promoter als source Enzymes Acetoin (g/L) BDO (g/L) Ethanol (g/L)
9C-BC9 pEZ-BC9 Ptet B. licheniformis Als, AldC, Bdh 1.6 ± 0.4 10.8 ± 1.1 26.2 ± 0.5
9C-BC10 pEZ-BC10 Ptet B. subtilis Als, AldC, Bdh 1.1 ± 0.5 10.2 ± 2.0 27.4 ± 1.6
9C-BC11 pEZ-BC11 Ptet B. subtilis Als, AldC, Bdh 0.7 ± 0.1 13.3 ± 0.7 24.9 ± 0.5
  1. The promoter of als gene in these constructed is driven by inducible promoter Ptet. Culture condition: 40 mL RMG8 with 200 μg/mL spectinomycin in 125-mL flask, 33 °C, 120 rpm. Four replicates each without tetracycline induction