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Table 4 Comparison of ATP requirements for C. thermocellum and model organisms during anaerobic growth

From: Exploring complex cellular phenotypes and model-guided strain design with a novel genome-scale metabolic model of Clostridium thermocellum DSM 1313 implementing an adjustable cellulosome

  ATP requirement (mmol ATP/g DCW)
C. thermocellum E. coli [46] S. cerevisiae [99]
Polysaccharides/cell wall 7.16 2.05 4.63
Protein
 Amino acid synthesis 5.65 1.36 1.81
 Polymerization 17.49 19.14 17.76
Lipid 0.52 0.14 0
RNA
 Nucleoside monophosphate formation 1.20 3.45 1.35
 Polymerization 0.27 0.92
 mRNA turnover 0.30 1.39 0.71
DNA
 Deoxynucleoside monophosphate formation 0.48 0.86  
 Polymerization 0.18 0.19  
 Subtotal 33.23 29.50 26.26
Transport
 Ammonium 8.43 4.24 6.29
 Potassium 0.20 0.19 2.4
 Phosphate 0.80 0.77
Total ATP required 42.66 34.71 34.95
Y MAXATP (g DCW/mol ATP) 23.44 28.81 28.61