Exploring complex cellular phenotypes and model-guided strain design with a novel genome-scale metabolic model of Clostridium thermocellum DSM 1313 implementing an adjustable cellulosome

Table 4 Comparison of ATP requirements for C. thermocellum and model organisms during anaerobic growth

	ATP requirement (mmol ATP/g DCW)
	C. thermocellum	E. coli [46]	S. cerevisiae [99]
Polysaccharides/cell wall	7.16	2.05	4.63
Protein
Amino acid synthesis	5.65	1.36	1.81
Polymerization	17.49	19.14	17.76
Lipid	0.52	0.14	0
RNA
Nucleoside monophosphate formation	1.20	3.45	1.35
Polymerization	0.27	0.92	1.35
mRNA turnover	0.30	1.39	0.71
DNA
Deoxynucleoside monophosphate formation	0.48	0.86
Polymerization	0.18	0.19
Subtotal	33.23	29.50	26.26
Transport
Ammonium	8.43	4.24	6.29
Potassium	0.20	0.19	2.4
Phosphate	0.80	0.77	2.4
Total ATP required	42.66	34.71	34.95
Y ^MAX_ATP (g DCW/mol ATP)	23.44	28.81	28.61

ISSN: 2731-3654