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Table 3 Plasmids used in this study

From: The synthetic xylulose-1 phosphate pathway increases production of glycolic acid from xylose-rich sugar mixtures

Name

Relevant characteristics

Reference

pCP20

ori pSC101, AmpR, plasmid expressing Flp recombinase to remove Kan cassette

[42]

pKD46

ori oriR101 w/repA101ts, AmpR, plasmid expressing λ Red recombinase genes

[18]

pKD3

ori R6 Kγ, CmR, source for cat cassette

[18]

pKD4

ori R6 Kγ, KanR, source for kan cassette

[18]

pACT3

ori p15A, CmR

[23]

pEXT20

ori colE1, AmpR

[23]

pGS

pACT3 derivative carrying ghrA and aceA genes from E. coli

This study

pX1P

pEXT20 derivative carrying human genes khk-c and codon optimized aldoB, and aldA from E. coli

[16]