Fig. 7

HxtB confers growth of S. cerevisiae in the presence of xylose. a HxtB::GFP localizes to the plasma membrane in S. cerevisiae when grown in maltose-rich media. Microscopy pictures were taken in the absence (DIC) and presence of fluorescence (GFP). b Growth of S. cerevisiae strain EBY.VW4000 transformed with (hxtB) and without (control) the hexose transporter hxtB gene in the presence of low (0.1 %) and high (1 %) concentrations of xylose. Both the control and hxtB strains also contained the genes encoding enzymes of the xylose metabolic pathway. Yeast strains were first pre-grown in maltose-rich media before a serial dilution was made and cells were incubated on plates containing the different carbon sources at 30 °C for 144 h. c Xylose transport as determined by [¹⁴C] xylose uptake in the S. cerevisiae hxtB strain in the presence of different concentrations of xylose. A S. cerevisiae strain which did not contain the HxtB hexose transporter was used as the control strain. Standard deviations were obtained from three biological replicates. d Growth, as determined by optical density at 600 nm (OD_600nm), ethanol production and xylose consumption of the S. cerevisiae control (does not contain hxtB) and HxtB strains when incubated in YNB supplemented with 1 % xylose