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Table 2 Strains and plasmids used in this work

From: Identification and characterization of putative xylose and cellobiose transporters in Aspergillus nidulans

Strains/plasmids

Genotype

Reference

S. cerevisiae

 EBY.VW4000

CEN.PK2-1C hxt13Δ::loxP hxt15Δ::loxP hxt16Δ::loxP hxt14Δ::loxP hxt12Δ::loxP hxt9Δ::loxP hxt11Δ::loxP hxt10Δ::loxP hxt8Δ::loxP hxt514Δ::loxP hxt2Δ::loxP hxt367Δ::loxP gal2 Δ stl1Δ::loxP agt1Δ::loxP ydl247wΔ::loxP yjr160cΔ::loxP

[48]

 SC9721

MATa his 3-D200 URA 3-52 leu2D1 lys 2D202 trp 1D63

FGSC

 EBY.VW4000 +pRH195m +pRH274

EBYVW4000 pRH195 pRH274

[33]

 hxtBGFP EBY.VW4000

EBYVW4000 pRH195 hxtB pRH274

This work

 xtrF::GFP EBY.VW4000

EBYVW4000 pRH195 xtrF pRH274

This work

 xtrG::GFP EBY.VW4000

EBYVW4000 pRH195 xtrG pRH274

This work

 xtrH::GFP EBY.VW4000

EBYVW4000 pRH195 xtrH pRH274

This work

 cltB::GFP EBY.VW4000

EBYVW4000 pRH195 cltB pRH274

This work

 SC9721 cltA::GFP gh1-1

SC9721 pRH195 cltA pGH1

This work

 SC9721 cltB::GFP gh1-1

SC9721 pRH195 cltB pGH1

This work

 SC9721_pGH1

SC9721 pGH1

This work

 SC9721_pCDT-1 gh1-1

SC9721 pCDT-1 pGH1

This work

A. nidulans

 TN02A3

pyroA4 pyrG89; chaA1; ΔnKuA::argB

[70]

 HxtB::GFP TN02A3

pyrG89; pyroA4; Δnku70::argB; hxtB::GFP::pyrG

This work

 HxtE::GFP TN02A3

pyrG89; pyroA4; Δnku70::argB; hxtE::GFP::pyrG

This work

 ΔxtrF

pyroA4 pyrG89; chaA1; ΔnKuA::argB; ΔxtrF::pyrG

This work

 ΔxtrG/ΔcltA

pyroA4 pyrG89; chaA1; ΔnKuA::argB; ΔxtrG::pyrG

This work

 ΔxtrH

pyroA4 pyrG89; chaA1; ΔnKuA::argB; ΔxtrH::pyrG

This work

 ΔcltB

pyroA4 pyrG89; chaA1; ΔnKuA::argB; ΔcltB::pyroA4

This work

 ΔhxtB

pyroA4 pyrG89; chaA1; ΔnKuA::argB; ΔhxtB::pyroA4

[46]

 ΔhxtE

pyroA4 pyrG89; chaA1; ΔnKuA::argB; ΔhxtE::pyroA4

[46]

 ΔcltA ΔcltB

pyroA4 pyrG89; chaA1; ΔnKuA::argB; ΔcltA::pyrG89; ΔcltB::pyroA4

This work

 GR5

wA1 pyroA1pyrG89

FGSC

 ΔcltB::cltB +

ΔcltB::cltB + ::pyrG + pyrG89

This work

 oCltB3

wA1 pyroA1 pyrG89 cltB3::gfp::pyrG +

This work

Plasmids

 pRH195a

pBluescript II SK+, TRP1, CEN6, ARSH4+ PHXT7-XKS1-THXT7

[24]

 pRH274

pBluescript II SK+, URA3, CEN6, ARSH4 + PPGK1-XYL1-TPGK1; PADH1-XYL2-TADH1; PHXT7-XKS1-THXT7

[66]

 pRS426

ampR lacZ URA3

[65]

 pCDA21

Zeo::pyr ampR

[71]

 pGH1-1

pRS425 PGK1p-gh1-1-CYC1t

[26]

 pCDT-1

pRS426 PGK1p-cdt-1-CYC1t

[26]

  1. aThe original vector pRH195 carries the XKS1 gene which was released after digestion with SpeI and SalI. The resultant vector without the XKS1 gene was used in this work for compl ementation assays