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Table 2 Strains and plasmids used in this work

From: Identification and characterization of putative xylose and cellobiose transporters in Aspergillus nidulans

Strains/plasmids Genotype Reference
S. cerevisiae
 EBY.VW4000 CEN.PK2-1C hxt13Δ::loxP hxt15Δ::loxP hxt16Δ::loxP hxt14Δ::loxP hxt12Δ::loxP hxt9Δ::loxP hxt11Δ::loxP hxt10Δ::loxP hxt8Δ::loxP hxt514Δ::loxP hxt2Δ::loxP hxt367Δ::loxP gal2 Δ stl1Δ::loxP agt1Δ::loxP ydl247wΔ::loxP yjr160cΔ::loxP [48]
 SC9721 MATa his 3-D200 URA 3-52 leu2D1 lys 2D202 trp 1D63 FGSC
 EBY.VW4000 +pRH195m +pRH274 EBYVW4000 pRH195 pRH274 [33]
 hxtBGFP EBY.VW4000 EBYVW4000 pRH195 hxtB pRH274 This work
 xtrF::GFP EBY.VW4000 EBYVW4000 pRH195 xtrF pRH274 This work
 xtrG::GFP EBY.VW4000 EBYVW4000 pRH195 xtrG pRH274 This work
 xtrH::GFP EBY.VW4000 EBYVW4000 pRH195 xtrH pRH274 This work
 cltB::GFP EBY.VW4000 EBYVW4000 pRH195 cltB pRH274 This work
 SC9721 cltA::GFP gh1-1 SC9721 pRH195 cltA pGH1 This work
 SC9721 cltB::GFP gh1-1 SC9721 pRH195 cltB pGH1 This work
 SC9721_pGH1 SC9721 pGH1 This work
 SC9721_pCDT-1 gh1-1 SC9721 pCDT-1 pGH1 This work
A. nidulans
 TN02A3 pyroA4 pyrG89; chaA1; ΔnKuA::argB [70]
 HxtB::GFP TN02A3 pyrG89; pyroA4; Δnku70::argB; hxtB::GFP::pyrG This work
 HxtE::GFP TN02A3 pyrG89; pyroA4; Δnku70::argB; hxtE::GFP::pyrG This work
 ΔxtrF pyroA4 pyrG89; chaA1; ΔnKuA::argB; ΔxtrF::pyrG This work
 ΔxtrG/ΔcltA pyroA4 pyrG89; chaA1; ΔnKuA::argB; ΔxtrG::pyrG This work
 ΔxtrH pyroA4 pyrG89; chaA1; ΔnKuA::argB; ΔxtrH::pyrG This work
 ΔcltB pyroA4 pyrG89; chaA1; ΔnKuA::argB; ΔcltB::pyroA4 This work
 ΔhxtB pyroA4 pyrG89; chaA1; ΔnKuA::argB; ΔhxtB::pyroA4 [46]
 ΔhxtE pyroA4 pyrG89; chaA1; ΔnKuA::argB; ΔhxtE::pyroA4 [46]
 ΔcltA ΔcltB pyroA4 pyrG89; chaA1; ΔnKuA::argB; ΔcltA::pyrG89; ΔcltB::pyroA4 This work
 GR5 wA1 pyroA1pyrG89 FGSC
 ΔcltB::cltB + ΔcltB::cltB + ::pyrG + pyrG89 This work
 oCltB3 wA1 pyroA1 pyrG89 cltB3::gfp::pyrG + This work
Plasmids
 pRH195a pBluescript II SK+, TRP1, CEN6, ARSH4+ PHXT7-XKS1-THXT7 [24]
 pRH274 pBluescript II SK+, URA3, CEN6, ARSH4 + PPGK1-XYL1-TPGK1; PADH1-XYL2-TADH1; PHXT7-XKS1-THXT7 [66]
 pRS426 ampR lacZ URA3 [65]
 pCDA21 Zeo::pyr ampR [71]
 pGH1-1 pRS425 PGK1p-gh1-1-CYC1t [26]
 pCDT-1 pRS426 PGK1p-cdt-1-CYC1t [26]
  1. aThe original vector pRH195 carries the XKS1 gene which was released after digestion with SpeI and SalI. The resultant vector without the XKS1 gene was used in this work for compl ementation assays