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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris

Fig. 2

Yield of the error-prone RCA and MDA products after digestion with restriction enzyme. Various amounts of plasmid (pGAPZα/cel6A) were amplified with MnCl2 (a), and the obtained error-prone RCA products were amplified without MnCl2 (b). Amplified DNAs were digested with restriction enzyme BlnI, which cleaves a single site in the vector. The DNA separated by agarose gel electrophoresis showed a single plasmid-sized band (4.4 kb)

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