Fig. 2From: Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris Yield of the error-prone RCA and MDA products after digestion with restriction enzyme. Various amounts of plasmid (pGAPZα/cel6A) were amplified with MnCl2 (a), and the obtained error-prone RCA products were amplified without MnCl2 (b). Amplified DNAs were digested with restriction enzyme BlnI, which cleaves a single site in the vector. The DNA separated by agarose gel electrophoresis showed a single plasmid-sized band (4.4 kb)Back to article page