Fig. 4From: Development of simple random mutagenesis protocol for the protein expression system in Pichia pastoris Histogram of amorphous cellulose (PASC)-degrading activities of transformants. a The activities of 87 transformants obtained by error-prone RCA-MDA under the conditions of 2 mM Mn2+ and 100 pg template. b The activities of 100 transformants obtained by error-prone RCA-MDA under the conditions of 2 mM Mn2+and 250 pg template. c The activities of 96 transformants obtained by the usual plasmids preparation protocol (wild-type control)Back to article page