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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Role of surface tryptophan for peroxidase oxidation of nonphenolic lignin

Fig. 3

SEC profiles of softwood (a) and hardwood (b) lignosulfonates treated for 24 h with native VP and its W164S variant and control without enzyme, and sulfonated polystyrene standards (c). Lignosulfonate samples (12 g L−1) after a 24-h treatment with 1.2 µM native VP (green line) and its W164S variant (dashes) in presence of 9.5 mM H2O2, and the corresponding softwood (red) and hardwood (blue) lignosulfonate controls without enzyme, were analyzed in a Superdex-75 column using 0.15 M NaOH as eluent (0.5 mL·min−1) and detection at 280 nm. Sulfonated polystyrenes (Mp 78,400, 29,500, 10,200 and 4210 Da, from left to right) were used as molecular mass standards in c (arrow shows the excluded blue dextran elution volume)

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