Table 2 CII reduction constants by acetylated, methylated and native (softwood and hardwood) lignosulfonates: Native VP vs W164S variant
From: Role of surface tryptophan for peroxidase oxidation of nonphenolic lignin
| Â | Softwood lignin | Hardwood lignin | ||||
|---|---|---|---|---|---|---|
Acetylated | Methylated | Native | Acetylated | Methylated | Native | |
Native VP | ||||||
k 3 (s−1) | 14.4 ± 0.4 | 21.2 ± 2.0 | 48 ± 2 | 12.2 ± 0.5 | 18.4 ± 1.6 | 14 ± 1 |
K D3 (μM) | 24.1 ± 1.9 | 147 ± 25 | 143 ± 19 | 20.6 ± 2.5 | 226 ± 33 | 14 ± 2 |
k 3app (s−1 mM−1) | 599 ± 31 | 144 ± 10 | 340 ± 30 | 592 ± 52 | 82 ± 5 | 990 ± 80 |
W164S variant | ||||||
k 3 (s−1) | – | – | – | 1.6 ± 0.2 | – | – |
K D3 (μM) | – | – | – | 98.2 ± 22.5 | – | – |
k 3app (s−1 mM−1) | 9.0 ± 0.8 | 3.0 ± 0.3 | 96 ± 12 | 16 ± 2 | 0.23 ± 0.07 | 8 ± 0.1 |