Table 1 Bacteria strains and plasmids used in this study

Strains

 E. coli DH5α [60]

F⁻(80d lacZ M15) (lacZYA-argF) U169 hsdR17(r⁻ m⁺) recA1 endA1 relA1 deoR96

 Amorphadiene-producing E. coli [39]

E. coli DH1 harboring pBbA5c-MevT-MBIS plasmid and pADS, Strain 2p

 S. elongatus PCC 7942

Wild type (ATCC 33912)

 SeHL11A

S. elongatus NSI::Bb1 s-dxs NSII::Bb2 k-ADS

 SeHL12A

S. elongatus PCC 7942 NSI::Bb1 s-idi, NSII::Bb2 k-ADS

 SeHL21A

S. elongatus PCC 7942 NSI::Bb1 s-dxs-dxr NSII::Bb2 k-ADS

 SeHL22A

S. elongatus NSI::Bb1 s-dxs-idi NSII::Bb2 k-ADS

 SeHL23A

S. elongatus PCC 7942 NSI::Bb1 s-dxr-idi NSII::Bb2 k-ADS

 SeHL31A

S. elongatus NSI::Bb1 s-dxs-dxr-idi NSII::Bb2 k-ADS

 SeHL32A

S. elongatus NSI::Bb1 s-dxr-idi-ispA NSII::Bb2 k-ADS

 SeHL33

S. elongatus NSI::Bb1 s-dxs-idi-ispA

 SeHL33A

S. elongatus NSI::Bb1 s-dxs-idi-ispA NSII::Bb2 k-ADS

 SeHL41A

S. elongatus NSI::Bb1 s-dxs-dxr-idi-ispA NSII::Bb2 k-ADS

 SeSC00S

S. elongatus NSII::Bb2 k-SQS

 SeSC33S

S. elongatus NSI::Bb1 s-dxs-idi-ispA NSII::Bb2 k-SQS

 SeSC41S

S. elongatus NSI::Bb1 s-dxs-dxr-idi-ispA NSII::Bb2 k-SQS

Plasmids^a

 pSyn_1 (Invitrogen)

pUC, Spc^r, P _Ni , NSI target sites

 pSe1Bb1 s-gfp [33]

pUC, Spc^r, LacI, P_trc, BglBrick sites, NSI targeting

SyneBrick vector: derived from pSyn_1 vector

 pSe2Bb1 k-gfp [33]

pUC, Km^r, LacI, P_trc, BglBrick sites, NSII targeting

SyneBrick vector

 pSe1Bb1 s-dxs

pUC, Spc^r, LacI, P_trc, dxs(se.co) NSI targeting

 pSe1Bb1 s-idi

pUC, Spc^r, LacI, P_trc, idi(se.co), NSI target site

 pSe1Bb1 s-dxs-idi

pUC, Spc^r, LacI, P_trc, dxs(se.co), idi(se.co), NSI targeting

 pSe1Bb1 s-dxs-dxr

pUC, Spc^r, LacI, P_trc, dxs(se.co), dxr(se.co), NSI target site

 pSe1Bb1 s-dxr-idi

pUC, Spc^r, LacI, P_trc, dxr(se.co), idi(se.co), NSI target site

 pSe1Bb1 s-dxs-dxr-idi

pUC, Spc^r, LacI, P_trc, dxs(se.co), dxr(se.co), idi(se.co), NSI target site

 pSe1Bb1 s-dxr-idi-ispA

pUC, Spc^r, LacI, P_trc, dxr(se.co), idi(se.co), ispA(se.co), NSI targeting

 pSe1Bb1 s-dxs-idi-ispA

pUC, Spc^r, LacI, P_trc, dxs(se.co), idi(se.co), ispA(se.co)

NSI targeting

 pSe1Bb1 s-dxs-dxr-idi-ispA

pUC, Spc^r, LacI, P_trc, dxs(se.co), dxr(se.co), idi(se.co), ispA(se.co), NSI targeting

 pSe2Bb1 k-ADS

pUC, Km^r, LacI, P_trc, ADS(se.co), NSII targeting

 pSe2Bb1 k-SQS

pUC, Km^r, LacI, P_trc, SQS(se.co), NSII targeting