Table 1 Strains and plasmids used in this study

Strains

 MG1655

E. coli K-12 F⁻λ⁻ ilvG ⁻ rfb-50rph-1

[40]

 SBF01

MG1655 with pFAB

This study

 SBF02

MG1655 containing pFAB and pBbB6c-‘tesA

This study

 SBF03

MG1655 with ΔfadE::FRT

This study

 SBF04

MG1655 with ΔfadE::FRT containing pFAB

This study

 SBF05

MG1655 with ΔfadE::FRT containing pFAB and pBbB6c-‘tesA

This study

 SBF06

MG1655 with pBbB6c-‘tesA

This study

 SBF07

MG1655 with pBbB6c-‘tesA ^{A120D, A171V}

This study

 SBF08

MG1655 with pBbB6c-‘tesA ^R64C

This study

 SBF09

MG1655 with pBbB6c-‘tesA ^D74G

This study

 SBF10

MG1655 with pBbB6c-‘tesA ^R64T

This study

 SBF11

MG1655 with pBbB6c-‘tesA ^R64Q

This study

 SBF12

MG1655 with pBbB6c-‘tesA ^FT

This study

 SBF13

MG1655 with pBbB6c-‘tesA ^R64CFT

This study

 SBL01

MG1655 library containing pFAB and mutated ‘TesA

This study

 BL21(DE3)

E. coli B F⁻ ompT gal dcm lon hsdS _B (r ⁻ _B m ⁻ _B ) λ(DE3 acI lacUV5-T7 gene 1 ind1 sam7 nin5])

[41]

 BL21(DE3)-‘TesA

BL21(DE3) with pET28a-‘tesA

This study

 BL21(DE3)-‘TesA^R64C

BL21(DE3) with pET28a-‘tesA ^R64C

This study

Plasmids

 pBbE8a-rfp

ColE1 ori, carrying P_BAD promoter and rfp, Amp^R

[42]

 pBbE8a-fadR

pBbE8a with Δrfp::fadR (from MG1655), Amp^R

This study

 pBBR1 MCS-3

Cloning vactor carrying MCS and lacZ alpha, Tc^R

[43]

 pFAB

pBbE8a-fadR carrying P_LR-tetA-rfp on AatII site

This study

 pBbB6c-gfp

BBR1 ori, carrying P_L-lacO1 promoter and gfp, Cm^R

[42]

 pBbB6c-‘tesA

pBbB6c-gfp with Δgfp::‘tesA, Cm^R

This study

 pBbB6c-‘tesA ^{A120D, A171V}

pBbB6c-gfp with Δgfp::‘tesA ^{A120D, A171V}, Cm^R

This study

 pBbB6c-‘tesA ^R64C

pBbB6c-gfp with Δgfp::‘tesA ^R64C, Cm^R

This study

 pBbB6c-‘tesA ^D74G

pBbB6c-gfp with Δgfp::‘tesA ^D74G, Cm^R

This study

 pBbB6c-‘tesA ^R64T

pBbB6c-gfp with Δgfp::‘tesA ^R64T, Cm^R

This study

 pBbB6c-‘tesA ^R64Q

pBbB6c-gfp with Δgfp::‘tesA ^R64Q, Cm^R

This study

 pBbB6c-‘tesA ^FT

pBbB6c- gfp with Δgfp::flag tagged‘tesA, Cm^R

This study

 pBbB6c-‘tesA ^R64CFT

pBbB6c- gfp with Δgfp::flag tagged‘tesA ^R64C, Cm^R

This study

 pET28a (+)

Expression vector with (His)₆-tag, Km^R

Novagen

 pET28a-‘tesA

pET28a with C-terminally (His)₆-tagged ‘tesA

This study

 pET28a-‘tesA ^R64C

pET28a with C-terminally (His)₆-tagged ‘tesA ^R64C

This study