Fig. 5From: Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproductionEffects of different combinations of glucose and glycerol as substrate and heterologous addition of pyruvic acid and dodecane on the production of limonene. a Glucose of 2, 1.5, 1, 0.5, 0 % were respectively combined with glycerol of 0, 0.5, 1, 1.5, 2 % as substrate in the YP medium of strain Po1f-LN-051. b Pyruvic acid was added to the YPD medium of strain Po1f-LN-051 to a final concentration of 0, 2 , 4, or 8 g/L. c Dodecane was added to the YPD medium of strain Po1f-LN-051 with 4 g/L pyruvic acid as the auxiliary carbon source and the proportion of dodecane was from 2 to 10 %. Three repeats were performed for each medium, and error bars represent standard deviations. t tests were conducted to evaluate statistical significance at p < 0.05. Particularly, for (a) the asterisk shows statistical significance between 2 % glucose and other combinations of glucose and glycerol, for (b) the asterisk shows statistical significance between YPD medium containing 0 g/L pyruvic acid and other concentrations of pyruvic acid, and for (c) the asterisk shows statistical significance between YPD medium containing 2 % dodecane and other proportions of dodecaneBack to article page