Table 1 Vectors used in this study
From: Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction
Vectors | Description | Source |
|---|---|---|
pINA1312 | Y. lipolytica-integrative plasmid, hp4d promoter, XPR2 terminator, ura3d1 selection marker, KmR | [22] |
pINA1269 | Y. lipolytica-integrative plasmid, hp4d promoter, XPR2 terminator, LEU2 selection marker, AmpR | [22] |
p1312-tLS | Constitutively expressed truncated LS gene | This study |
p1312-tNDPS1 | Constitutively expressed truncated NDPS1 gene | This study |
p1312wLN | Constitutively expressed tLS and tNDPS1 genes | This study |
p1312LN | Constitutively expressed codon-optimized tLS and codon-optimized tNDPS1 genes | This study |
p1269tHMG1 | Constitutively expressed tHMG1 gene | This study |
p1269HMG1 | Constitutively expressed HMG1 gene | This study |
p1269IDI1 | Constitutively expressed IDI1 gene | This study |
p1269ERG8 | Constitutively expressed ERG8 gene | This study |
p1269ERG10 | Constitutively expressed ERG10 gene | This study |
p1269ERG12 | Constitutively expressed ERG12 gene | This study |
p1269ERG19 | Constitutively expressed ERG19 gene | This study |
p1269tLS | Constitutively expressed tLS gene | This study |
p1269tNPDS1 | Constitutively expressed tNDPS1 gene | This study |
p1269tHMG1tHMG1 | Constitutively expressed tHMG1 gene of two copies | This study |
p1269tHMG1tHMG1tHMG1 | Constitutively expressed tHMG1 gene of three copies | This study |
p1269HMG1HMG1 | Constitutively expressed HMG1 gene of two copies | This study |
p1269IDI1IDI1 | Constitutively expressed IDI1 gene of two copies | This study |
p1269IDI1IDI1IDI1 | Constitutively expressed IDI1 gene of three copies | This study |
p1269HMG1IDI1 | Constitutively expressed HMG1 and IDI1 genes | This study |
p1269HMG1ERG8 | Constitutively expressed HMG1 and ERG8 genes | This study |
p1269HMG1ERG10 | Constitutively expressed HMG1 and ERG10 genes | This study |
p1269HMG1ERG12 | Constitutively expressed HMG1 and ERG12 genes | This study |
p1269HMG1ERG19 | Constitutively expressed HMG1 and ERG19 genes | This study |
p1269HMG1tLS | Constitutively expressed HMG1 and tLS genes | This study |
p1269HMG1tNPDS1 | Constitutively expressed HMG1 and tNDPS1 genes | This study |