Table 3 Primers used in this study
From: Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction
Primer | Primer sequence (5′–3′) |
|---|---|
P1 | ACAACCACACACATCCACGTGATGCGACGATCCGGTAACTACTCCCCTT (PmlI) |
P2 | TTAGTTTCGGGTTCCCACGTGCTAGGCGAAAGGCTGGAACAGGCAA (PmlI) |
P3 | ACAACCACACACATCCACGTGATGTCCGCCCGAGGTCTCAACAAAA (PmlI) |
P4 | TTAGTTTCGGGTTCCCACGTGCTAGTAGGTGTGGCCACCGAATCGT (PmlI) |
P5 | AGATAGAGTCGACAAAGGCCTGCTAGCTTATCGATACGCGTGCATG (StuI) |
P6 | TGTACACCGAGAAACAGGCCTCATCTCACTTGCGTATGTATGGAAA (StuI) |
P7 | ACAACCACACACATCCACGTGATGCTACAAGCAGCTATTGG (PmlI) |
P8 | TTAGTTTCGGGTTCCCACGTGCTATGACCGTATGCAAATATT (PmlI) |
P9 | ACAACCACACACATCCACGTGATGACGACGTCTTACAGCGA (PmlI) |
P10 | TTAGTTTCGGGTTCCCACGTGCTACTTGATCCACCGCCGAA (PmlI) |
P11 | ACAACCACACACATCCACGTGATGACCACCTATTCGGCTCC (PmlI) |
P12 | TTAGTTTCGGGTTCCCACGTGCTACTTGAACCCCTTCTCGA (PmlI) |
P13 | ACAACCACACACATCCACGTGATGCGACTCACTCTGCCCCG (PmlI) |
P14 | TTAGTTTCGGGTTCCCACGTGCTACTCGACAGAAGAGACCT (PmlI) |
P15 | ACAACCACACACATCCACGTGATGGACTACATCATTTCGGC (PmlI) |
P16 | TTAGTTTCGGGTTCCCACGTGCTAATGGGTCCAGGGACCGA (PmlI) |
P17 | ACAACCACACACATCCACGTGATGATCCACCAGGCCTCCAC (PmlI) |
P18 | TTAGTTTCGGGTTCCCACGTGCTACTTGCTGTTCTTCAGAG (PmlI) |
P19 | CGAGGCAGCAGATCCACTAGTAGCACCGCCGCCGCAAGGAATGG (SpeI) |
P20 | GCGGCCGCATAGGCCACTAGTCTGTCAAACATGAGAATTCGG (SpeI) |
P21 | CTCTCAAGGGCATCGGTCGACAGCACCGCCGCCGCAAGGAATGG (SalI) |
P22 | CGCATAAGGGAGAGCGTCGACCTGTCAAACATGAGAATTCGG (SalI) |
P23 | CGGCATCCGCTTACAGAC |
P24 | GGAGGCATCAGTGACCAAA |
P25 | CATTAGGAAGCAGCCCAGTA |
P26 | GAGATCGTCAAGGGTTTG |
P27 | CATAAGTGCGGCGACGAT |
P28 | CTACTACTGGGCTGCTTCCTA |