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Table 1 Comparison of ratios calculated from iTRAQ proteomics and RT-PCR analyses

From: Proteomic and metabolomic analyses reveal metabolic responses to 3-hydroxypropionic acid synthesized internally in cyanobacterium Synechocystis sp. PCC 6803

 

Gene ID

Proteomics ratio

RT-PCR ratio

Function description

Up-regulated

slr0420

2.76 ± 0.05

4.05 ± 1.88

Hypothetical protein

sll1773

2.51 ± 0.05

4.24 ± 1.65

Conserved hypothetical protein

slr0431

2.42 ± 0.09

2.61 ± 0.83

Conserved hypothetical protein

slr1227

2.02 ± 0.36

0.85 ± 0.15

OMP1 precursor

sll1869

1.71 ± 0.16

2.71 ± 0.46

A-subunit oxygenase

sll0385

1.35 ± 0.02

0.90 ± 0.46

ABC transporter

sll1699

1.34 ± 0.04

2.22 ± 0.34

Extracellular solute binding

slr0844

1.37 ± 0.06

0.88 ± 0.25

NADH-plastoquinone oxidoreductase chain 5

sll1598

1.24 ± 0.02

1.73 ± 0.57

Iron (chelated) ABC transporter,

sll1491

1.32 ± 0.49

4.35 ± 0.03

Periplasmic WD repeat-containing protein

Down-regulated

sll0992

0.42 ± 0.01

0.80 ± 0.08

Conserved hypothetical protein

ssl2667

0.51 ± 0.03

0.68 ± 0.05

Putative NifU protein

ssr2061

0.44 ± 0.03

0.65 ± 0.09

Glutaredoxin 3 (grx3)

slr0447

0.49 ± 0.02

0.68 ± 0.23

Urea/short-chain amide ABC transporter

sll0541

0.65 ± 0.13

0.75 ± 0.29

Delta-9 desaturase

slr1019

0.56 ± 0.08

1.75 ± 0.35

PhzF

sll0482

0.75 ± 0.21

2.06 ± 0.98

Lipase precursor

slr1856

0.79 ± 0.11

2.21 ± 0.57

Sigma regulatory factor

sll1087

0.83 ± 0.10

0.89 ± 0.32

Sodium-coupled permease

slr1200

0.75 ± 0.05

1.08 ± 0.20

Urea/short-chain amide ABC transporter, permease protein