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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Examining Escherichia coli glycolytic pathways, catabolite repression, and metabolite channeling using Δpfk mutants

Fig. 3

Glucose and acetate coutilization (n = 2). The growth (open square) and substrate consumption (glucose, open circle; acetate, open triangle) in mutant strains initially fed 10 g/L of glucose then supplemented with 6 g/L of sodium acetate (denoted in figure): apfkA (JW3887) and b WH04. OD600 of cultures grown solely with 10 g/L of glucose are also included (closed circles). c Maximal growth rate of WT, JW3887, and WH04 in cultures supplemented with 6 g/L of sodium acetate during early exponential phase (black bar). Control cultivations (no acetate; white bar) were supplemented with 6 g/L of sodium chloride to normalize for ion effects. d 13C tracing in cultivations fed 10 g/L of unlabeled glucose and 6 g/L of fully labeled 13C2-acetate. Acetate contribution (13C fraction) in proteinogenic amino acids are shown for the three strains

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