Parameter | Cultivation Conditions | ||||
---|---|---|---|---|---|
#1 | #2 | #3 | #4 | #5 | |
Total duration (h) | 30 | 50 | 50 | 40 | 40 |
Batch phase duration (h) | 6.0 ± 0.4 | 6.2 ± 0.3 | 9.5 ± 0.6 | 10.1 ± 0.9 | 10.4 ± 0.9 |
Initial glucose conc. (g L−1) | 5 | 5 | 20 | 20 | 20 |
OD600 at which fed-batch phase started | 8.1 ± 0.6 | 8.0 ± 0.4 | 22.1 ± 1.2 | 23.2 ± 2.6 | 23.7 ± 2.1 |
Induction time point (h) | 12.5 ± 0.9 | 10.5 ± 0.7 | 16.2 ± 1.1 | 15.9 ± 1.4 | 15.3 ± 1.5 |
OD600 of induction | 68.1 ± 5.1 | 45.0 ± 2.8 | 81.3 ± 4.9 | 79.6 ± 7.3 | 80.4 ± 7.0 |
IPTG conc. (mM) | 0.5 | 0.5 | 0.1 | 0.1 | 0.05 |
Propionate conc. (mM) | 40 | 40b | 60b | 60b | 60b |
n-Octanol conc. (mM) | 2 | 2c | 3c | 3c | 3c |
Oleic acid supply (mM) | 0.14a | 0.21a | 0.21a | 0.21a | 0.21a |
Post-induction growth rate (h−1) | 0.04 | 0.02 | 0.04 | 0.04 | 0.04 |
Post-induction temperature (°C) | 22 | 22 | 22 | 30 | 25 |
Maximum OD600 | 124.1 ± 8.9 | 74.0 ± 4.1 | 209.8 ± 12.4 | 142.1 ± 13.9 | 121.3 ± 11.0 |
Final OD600 | 114.3 ± 8.1 | 54.3 ± 2.9 | 199.2 ± 12.1 | 122.8 ± 11.1 | 97.0 ± 8.9 |
Productivity (mg MBE (L h)−1) | 13.7 ± 0.9 | 5.9 ± 0.3 | 15.8 ± 1.1 | 5.3 ± 0.6 | 10.9 ± 0.8 |
Yield of MBE (mg MBE (g glucose)−1) | 2.61 ± 0.1 | 2.13 ± 0.1 | 2.09 ± 0.2 | 0.98 ± 0.1 | 2.51 ± 0.3 |
Final Titer (mg MBE (L)−1) | 411.5 ± 5.5 | 295.3 ± 3.7 | 790.2 ± 6.9 | 212.1 ± 1.8 | 436.8 ± 3.9 |