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Table 1 Cultivation of E. coli RQ5/pMB07 under different fed-batch fermentations conditions

From: High cell density production of multimethyl-branched long-chain esters in Escherichia coli and determination of their physicochemical properties

Parameter

Cultivation Conditions

#1

#2

#3

#4

#5

Total duration (h)

30

50

50

40

40

Batch phase duration (h)

6.0 ± 0.4

6.2 ± 0.3

9.5 ± 0.6

10.1 ± 0.9

10.4 ± 0.9

Initial glucose conc. (g L−1)

5

5

20

20

20

OD600 at which fed-batch phase started

8.1 ± 0.6

8.0 ± 0.4

22.1 ± 1.2

23.2 ± 2.6

23.7 ± 2.1

Induction time point (h)

12.5 ± 0.9

10.5 ± 0.7

16.2 ± 1.1

15.9 ± 1.4

15.3 ± 1.5

OD600 of induction

68.1 ± 5.1

45.0 ± 2.8

81.3 ± 4.9

79.6 ± 7.3

80.4 ± 7.0

IPTG conc. (mM)

0.5

0.5

0.1

0.1

0.05

Propionate conc. (mM)

40

40b

60b

60b

60b

n-Octanol conc. (mM)

2

2c

3c

3c

3c

Oleic acid supply (mM)

0.14a

0.21a

0.21a

0.21a

0.21a

Post-induction growth rate (h−1)

0.04

0.02

0.04

0.04

0.04

Post-induction temperature (°C)

22

22

22

30

25

Maximum OD600

124.1 ± 8.9

74.0 ± 4.1

209.8 ± 12.4

142.1 ± 13.9

121.3 ± 11.0

Final OD600

114.3 ± 8.1

54.3 ± 2.9

199.2 ± 12.1

122.8 ± 11.1

97.0 ± 8.9

Productivity (mg MBE (L h)−1)

13.7 ± 0.9

5.9 ± 0.3

15.8 ± 1.1

5.3 ± 0.6

10.9 ± 0.8

Yield of MBE (mg MBE (g glucose)−1)

2.61 ± 0.1

2.13 ± 0.1

2.09 ± 0.2

0.98 ± 0.1

2.51 ± 0.3

Final Titer (mg MBE (L)−1)

411.5 ± 5.5

295.3 ± 3.7

790.2 ± 6.9

212.1 ± 1.8

436.8 ± 3.9

  1. Batch phase duration, OD600 values and productivities are expressed as media ± SD of three independent fermentations carried out for each condition
  2. aTotal quantity added in pulses of 0.07-mM oleic acid every 12-h post-induction
  3. bTotal quantity added in pulses of 20-mM propionate every 12-h post-induction
  4. cTotal quantity added in pulses of 1-mM n-octanol every 12-h post-induction