Fig. 6

The crude lipids and total lipids (minus chlorophyll a and b and total carotenoids) of Dunaliella tertiolecta cultured outdoors under different sodium bicarbonate concentrations in ATCC-1174 DA medium using rotating floating photobioreactor. a The changes in crude lipid concentrations of D. tertiolecta culture under 0.02 mol L⁻¹ NaHCO₃ (red bar) and 0.10 mol L⁻¹ NaHCO₃ (blue bar) as compared to the control, 0.00 mol L⁻¹ NaHCO₃ (black bar). b The changes in crude lipid productivity (g m⁻² day⁻¹) of D. tertiolecta culture under cultivation conditions as mentioned in a. c The changes in total lipid concentration of D. tertiolecta culture under 0.02 mol L⁻¹ NaHCO₃ (green bar) and 0.10 mol L⁻¹ NaHCO₃ (yellow bar) as compared to the control, 0.00 mol L⁻¹ NaHCO₃ (white bar). d The changes in total lipid productivity (g m⁻² day⁻¹) of D. tertiolecta culture under cultivation conditions mentioned in c. The negative values of productivities calculated in control condition were all shown as 0 g m⁻² day⁻¹. Data were expressed as mean ± standard deviation of three independent experiments (n = 3). ABC, abcd, and αβ indicated significant differences among different cultivation days under the control, Treatment 1, and Treatment 2, respectively. XYZ showed significant differences among control and different concentrations of sodium bicarbonate under the same cultivation day (one-way ANOVA; Tukey multiple comparison; p < 0.05)