Fig. 4

Archaeal and bacterial DGGE profiles and heat maps. Archaeal (a) and bacterial (c) DGGE profiles and heat maps of the relative intensities of major archaeal (b) and bacterial (d) DGGE bands. Numbered bands in a indicate the positions identical to the migration of clone samples closely related to (1–3) Methanosaeta concilii, (4) Methanobacterium formicicum, (5) Methanoregula boonei and/or Methanosarcina acetivorans, and (6) Methanoregula boonei and/or Methanobacterium formicicum. Numbered bands in b indicate the positions identical to the migration of clone samples closely related to (1) Brachymonas denitrificans and Tessaracoccus (2) Propionibacteriaceae, (3) Treponema, (4) Bacteroidales, (5) Bacteroidales and Victivallis, (6) Succiniclasticum, (7) Propioniferax, (8) Petrimonas, (9) Synergistaceae, Brachymonas denitrificans and Tessaracoccus, (10) Kosmotoga, (11) Clostridium quinii and Clostridia, and (12) Syntrophobacter fumaroxidans. Each band in c and d is labelled with the clone(s) with an identical migration pattern, followed in parentheses by the affiliation of the clone determined by Ribosomal Database Project classifier. Numbers indicate ratio (%) over the sum of band intensities of each sample (i.e., each lane in DGGE). P1–P6 and II, IV indicate operational periods and experiments described in Table 1