Table 1 Purification of a raw starch-digesting enzyme from P. oxalicum GXU20
Purification step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Fold purification |
|---|---|---|---|---|---|
Crude supernatant | 936.81 | 5233.64 | 0.18 | 100.00 | 1.00 |
Ethanol precipitation | 501.28 | 418.05 | 1.20 | 53.51 | 6.67 |
Hiprep 16/10 Phenyl FF | 314.44 | 127.28 | 2.47 | 33.56 | 13.72 |
Source 15S 4.6/100 PE | 108.68 | 9.96 | 10.91 | 11.60 | 60.61 |