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Table 5 Substrate specificity of an rPoGA15A mutant without SBD towards different starches when compared to the wild-type enzyme rPoGA15A

From: Efficient hydrolysis of raw starch and ethanol fermentation: a novel raw starch-digesting glucoamylase from Penicillium oxalicum

Substrate

Specific activity for wild-type enzyme rPoGA15A (U/mg protein)

Specific activity for mutant enzyme rPoGA15A lacking SBD (U/mg protein)

Relative activity (%)a

Raw cassava starch

11.2 ± 0.9

5.3 ± 0.2

47.4 ± 1.7

Raw rice starch

24.7 ± 0.4

4.5 ± 0.1

18.2 ± 0.5

Raw corn starch

22.7 ± 0.7

6.8 ± 0.1

30.3 ± 0.3

Raw potato starch

9.9 ± 0.5

5.1 ± 0.2

51.2 ± 1.4

Soluble starch

80.5 ± 0.7

57.9 ± 0.4

71.9 ± 0.5

  1. aValues were shown as percentages, which represent the substrate specific activity of the mutant enzyme rPoGA15A without SBD × 100/the corresponding substrate specific activity of the wild-type enzyme rPoGA15A
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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