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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Genome editing of Clostridium autoethanogenum using CRISPR/Cas9

Fig. 1

CRISPR/Cas9-based editing of C. autoethanogenum. a, b show design and screening for editing adh and 2,3-bdh, respectively. The modifications in the design for editing 2,3-bdh such as the position of the homology arms (5′HA and 3′HA, checkered gray box) relative to Cas9 cleavage site (T1 and T2; scissors) and cas9-inducible expression from IPL12 are highlighted in red. The alignment of sequence from Sanger sequencing of three clones (aadh clone T1.1, ∆adh clone T1.2 and b2,3-bdh clone T2.1) and next-generation sequencing (MiSeq; B, ∆2,3-bdh clone T1.2) confirms the partial deletion in adh and expected deletion in 2,3-bdh

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