Table 1 List of plasmids used in this study
From: Genome editing of Clostridium autoethanogenum using CRISPR/Cas9
Plasmid | Features | References |
|---|---|---|
pLZtet3no | Original tet3no promoter derived from Clostridium base vector pMTL82251 with pBP1 replicon, ermB marker and catP as a reporter gene | [23] |
pTet3no-cas9 | Cas9 between NdeI and NheI sites in pLZtet3no | This study |
pIPL1 | Tet3no promoter in pLZtet3no is replaced with IPL1 promoter | This study |
pIPL2 | Tet3no promoter in pLZtet3no is replaced with IPL2 promoter | This study |
pIPL3 | Tet3no promoter in pLZtet3no is replaced with IPL3 promoter | This study |
pIPL5 | Tet3no promoter in pLZtet3no is replaced with IPL5 promoter | This study |
pIPL8 | Tet3no promoter in pLZtet3no is replaced with IPL8 promoter | This study |
pIPL11 | Tet3no promoter in pLZtet3no is replaced with IPL11 promoter | This study |
pIPL12 | Tet3no promoter in pLZtet3no is replaced with IPL12 promoter | This study |
pIPL12-cas9 | Cas9 between NdeI and NheI sites in pIPL12 | This study |
pMTL83157 | pMTL83151 vector PWL promoter from C. autoethanogenum | [24] |
pgRNA-adh-T1 | pMTL83157 with gRNA T1 targeting adh gene | This study |
pgRNA-adh-T2 | pMTL83157 with gRNA T2 targeting adh gene | This study |
pgRNA-2,3bdh-T1 | pMTL83157 with gRNA T1 targeting 2,3bdh gene | This study |
pgRNA-2,3bdh-T2 | pMTL83157 with gRNA T2 targeting 2,3bdh gene | This study |
pgRNA-adh-T1_HA | pMTL83157 with gRNA T1 targeting adh gene with homology arms | This study |
pgRNA-adh-T2_HA | pMTL83157 with gRNA T2 targeting adh gene with homology arms | This study |
pgRNA-2,3bdh-T1_HA | pMTL83157 with gRNA T1 targeting 2,3bdh gene with homology arms | This study |
pgRNA-2,3bdh-T2_HA | pMTL83157 with gRNA T2 targeting 2,3bdh gene with homology arms | This study |