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Table 1 List of plasmids used in this study

From: Genome editing of Clostridium autoethanogenum using CRISPR/Cas9

Plasmid Features References
pLZtet3no Original tet3no promoter derived from Clostridium base vector pMTL82251 with pBP1 replicon, ermB marker and catP as a reporter gene [23]
pTet3no-cas9 Cas9 between NdeI and NheI sites in pLZtet3no This study
pIPL1 Tet3no promoter in pLZtet3no is replaced with IPL1 promoter This study
pIPL2 Tet3no promoter in pLZtet3no is replaced with IPL2 promoter This study
pIPL3 Tet3no promoter in pLZtet3no is replaced with IPL3 promoter This study
pIPL5 Tet3no promoter in pLZtet3no is replaced with IPL5 promoter This study
pIPL8 Tet3no promoter in pLZtet3no is replaced with IPL8 promoter This study
pIPL11 Tet3no promoter in pLZtet3no is replaced with IPL11 promoter This study
pIPL12 Tet3no promoter in pLZtet3no is replaced with IPL12 promoter This study
pIPL12-cas9 Cas9 between NdeI and NheI sites in pIPL12 This study
pMTL83157 pMTL83151 vector PWL promoter from C. autoethanogenum [24]
pgRNA-adh-T1 pMTL83157 with gRNA T1 targeting adh gene This study
pgRNA-adh-T2 pMTL83157 with gRNA T2 targeting adh gene This study
pgRNA-2,3bdh-T1 pMTL83157 with gRNA T1 targeting 2,3bdh gene This study
pgRNA-2,3bdh-T2 pMTL83157 with gRNA T2 targeting 2,3bdh gene This study
pgRNA-adh-T1_HA pMTL83157 with gRNA T1 targeting adh gene with homology arms This study
pgRNA-adh-T2_HA pMTL83157 with gRNA T2 targeting adh gene with homology arms This study
pgRNA-2,3bdh-T1_HA pMTL83157 with gRNA T1 targeting 2,3bdh gene with homology arms This study
pgRNA-2,3bdh-T2_HA pMTL83157 with gRNA T2 targeting 2,3bdh gene with homology arms This study
  1. The table also includes the main features of plasmids