Skip to main content

Table 2 List of oligonucleotides used in this study

From: Genome editing of Clostridium autoethanogenum using CRISPR/Cas9

Oligonucleotide

Sequence (5′ → 3′)

Purpose

Og01

GATTATAAGCGGCCGGCCATAAACTATTTTTTAAAGATAAAAGCT

PCR amplification of 5′-HA of adh

Og02

TACGCCGCCAGGTTTAACCAAAACCAGCTTGGACTTCTAAAGA

PCR amplification of 5′-HA of adh

Og03

TCTTTAGAAGTCCAAGCTGGTTTTGGTTAAACCTGGCGGCGTA

PCR amplification of 3′-HA of adh

Og04

CAACTTGCCCACTGGCCGGCCTGACTATTTCACTATGAGTAAATGGT

PCR amplification of 3′-HA of adh

Og05

GAATGTGTTTAAACTCTCTGAAACTAGCAAATTTGG

PCR amplification of 5′-HA of 2,3bdh

Og06

GAGATAATTATGAAAGCTGTATTGTGGTTGTAAAAGAAGGATTTGAAACAC

PCR amplification of 5′-HA of 2,3bdh

Og07

GTGTTTCAAATCCTTCTTTTACAACCACAATACAGCTTTCATAATTATCTC

PCR amplification of 3′-HA of 2,3bdh

Og08

AAAGGAGTTTAAACGAAAGTGAGCTTTTTGGTTATGAAAA

PCR amplification of 3′-HA of 2,3bdh

Og09

TATTAACCTTATAAAGTCCTACCCC

For screening

Og10

TAATCCTCCTCTTATAGTTTTGTGA

For screening

Og11

CAAAAGCTATACTTAGTGCTAGATT

For screening

Og12

TCATTTCTCTATCTTCAAAAAGTGT

For screening

Og13

AGCTGTAGATAACAATGGGATCAT

For screening

Og14

GTGAGATATAATGAGAAACCTGATCC

For screening

Og15

AATGGCAGGGCAGATAATTGTAATG

For screening

Og16

AAGGCATTCTGAGCCAGTTCTTTTA

For screening

Og17

TAACGTCCTTAACTATTTATCAATTCGATCGACTAT

To construct variants of ptet3no

  1. The table also includes the purpose of each oligonucleotide