Fig. 7

Amino acid alignment of soybean ferritin SferH-1 with Arabidopsis ferritins and selection of specific peptide that can distinguish SferH-1 from the endogenous host Arabidopsis ferritins. The first 49 amino acids of SferH-1 (GenBank accession no. M64337) is the transit peptide (TP) for delivery to plastids and is excised in the mature protein, thus it is excluded from the alignment. Arabidopsis has four ferritin genes that include from AtFER1 to AtFER4 (At5g01600, At3g11050, At3g56090, and At2g40300, respectively). The selected peptide KKSELAVPTAPQVS is predicted to be antigenic and highlighted in boldface. The underlined peptide is the segment that was used by Kanobe et al. [44], for preparing anti-soybean ferritin antibody. The boxed peptide sequence was picked by Murgia et al. [58], for Arabidopsis ferritin antibody preparation