Fig. 1

Development of transgenic Jatropha curcas plants expressing AtDGAT1. A T-DNA region (6.2 kb) of pBI121CaMV35S::AtDGAT plasmid. RB right border, LB left border, CaMV 35S promoter Nos P nopaline synthase promoter, Nos T nopaline synthase terminator, NptII neomycin phosphotransferase, ß-glucuronidase. B Agrobacterium-mediated genetic transformation of Jatropha curcas with 35S::AtDGAT construct: a excised cotyledonary leaf explants subjected to Agrobacterium tumefaciens-mediated transformation and cultured on kanamycin-free callus induction medium (bar 5 mm); b formation of callus from explant (bar 8 mm); c Stable GUS expression in buds induced from transformed callus (bar 8 mm); d untransformed control callus (bar 8 mm); e elongated putative transformed shoots on kanamycin selection (bar 1 cm); f rooted transformed plantlet (bar  1 cm); g acclimatized transformed plant. C Stable gus expression in transgenic plant tissues. a transformed cotyledonary leaf segment; b untransformed leaf segment; c emerging shoots from callus showing gus expression; d callus from untransformed tissues; e gus expression in in vitro shoot; f gus expression in leaf; g control leaf; h gus expression in stem; i control stem; j gus expression in transgenic root and k control root; l gus expression in transgenic seeds and m, control seed. (Bar 0.5 cm)