Fig. 5
From: Comparison of aldehyde-producing activities of cyanobacterial acyl-(acyl carrier protein) reductases
Western blotting of the supernatant and pellet fractions of the E. coli cell culture lysates. The E. coli cell culture coexpressing AAR and 73102 ADO was sonicated and centrifuged to separate the supernatant (S) and pellet (P) fractions. M denotes the molecular weight markers. The bands for AAR (38.6–39.8 kDa) and ADO (27.4 kDa) are indicated by arrows. The results for E. coli transformed with the pETDuet-1 vector containing neither the AAR nor ADO gene are shown in lanes 17 and 18 as a control. In a the coloring reaction lasted for ~2 min, while in b the reaction lasted more than 10 min for detection of low amounts of the soluble form of 7421 AAR, 73102 AAR, and 7336 AAR