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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Development of a modularized two-step (M2S) chromosome integration technique for integration of multiple transcription units in Saccharomyces cerevisiae

Fig. 1

Modular strategy and construction of Step 1 assembly procedure. a Structure of head-to-head promoter module; b Structure of terminator module for Step 1 assembly; c Structure of modularized gene coding sequence for Step 1 assembly; d Structure of the two-transcription-unit module from Step 1 assembly; e Digestion characteristic of Type IIs restriction enzymes. Letter N represents a random base; and colored region represents remaining DNA fragments by digestion of enzyme

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