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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Development of a modularized two-step (M2S) chromosome integration technique for integration of multiple transcription units in Saccharomyces cerevisiae

Fig. 4

Structure and identification strategy of the assembled and integrated β-carotene biosynthetic pathway. a Structure and identification strategy of the assembled and integrated β-carotene biosynthetic pathway in YPRCΔ15 site, F1’ to F5’ indicate five PCRs designed to confirm correct assembly and integration; b Gel picture of identification PCRs for assembly and integration. c HPLC analysis for β-carotene production

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