Fig. 4From: Development of a modularized two-step (M2S) chromosome integration technique for integration of multiple transcription units in Saccharomyces cerevisiae Structure and identification strategy of the assembled and integrated β-carotene biosynthetic pathway. a Structure and identification strategy of the assembled and integrated β-carotene biosynthetic pathway in YPRCΔ15 site, F1’ to F5’ indicate five PCRs designed to confirm correct assembly and integration; b Gel picture of identification PCRs for assembly and integration. c HPLC analysis for β-carotene productionBack to article page