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Table 1 Standardized adaptor sequence of individual gene for Golden Gate

From: Development of a modularized two-step (M2S) chromosome integration technique for integration of multiple transcription units in Saccharomyces cerevisiae

 

BsaI

SapI

BsmBI

Gene1 forward primer

GGTCTC

a GATG*

GCTCTTC

a GAT

CGTCTCA

a GATG

Gene1 reverse primer

GGTCTC

a AGGT

GCTCTTC

a AGG

CGTCTCA

a AGGT

Gene2 forward primer

GGTCTC

a GAAT

GCTCTTC

a GAA

CGTCTCA

a GAAT

Gene2 reverse primer

GGTCTC

a CGAT

GCTCTTC

a CGA

CGTCTCA

a CGAT

  1. *Italic 6–7 base sequences are recognition sites; Bold 4 or 3 base sequences are overhang sites. All sequences are written from 5′ to 3′