Table 2 Lists vectors used in this study
Plasmid | Genotypes | Sources |
|---|---|---|
pMD-19T | Amp+ | GENEWIZ |
pUC57-Kan | Kan+ | GENEWIZ |
P1(pTDH3-pADH) | Amp+ promoter vector with promoters TDH3 and ADH1 | This study |
P2(pPGK1-pTEF2) | Amp+ promoter vector with promoters PGK1 and TEF2 | This study |
P3(pFBA-pHXT7) | Amp+ promoter vector with promoters FBA and HXT7 | This study |
P4(pTPI1-pTEF1) | Amp+ promoter vector with promoters TPI1 and TEF1 | This study |
P5(pPDC1-pPYK1) | Amp+ promoter vector with promoters PDC1 and PYK1 | This study |
T1-(tTPI1-PGIt) | Amp+ terminator vector, contains terminators TPI1 and PGI | This study |
T2-(tADH1-tCYC1) | Amp+ terminator vector with terminators ADH1 and CYC1 | This study |
T3-(tFBA1-tPDC1) | Kan+ terminator vector with terminators FBA1 and PDC1 | This study |
T4-(tRPS2-tTDH1) | Kan+ terminator vector with terminators RPS2 and TDH1 | This study |
T5-(tCCW12-tRPL9A) | Kan+ terminator vector with terminators CCW12 and RPL9A | This study |
pKna(15site-Ura3) | Kan+ selective marker and integration locus | This study |
pKna(17site-His3) | Kan+ selective marker and integration locus | This study |
pKna(22site-Leu2) | Kan+ selective marker and integration locus | This study |