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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Production of l(+)-lactic acid from acid pretreated sugarcane bagasse using Bacillus coagulans DSM2314 in a simultaneous saccharification and fermentation strategy

Fig. 2

Simultaneous saccharification and fermentation (SSF) experiments performed using 200 g/l acid-pretreated bagasse DW and 10.5–15.8 FPU/g DW bagasse GC220 enzyme cocktail in pH controlled fermentations with a start volume of 1 l. As inoculum, either furfural-containing or reference preculture was used. The bagasse was either added in one batch or in two stages. Lactic acid production followed by calcium hydroxide addition, verified with HPLC measurements. The following experiments were performed: batch SSF solubilized with liquid fraction, inoculated with reference preculture (dashed line with dots), batch SSF solubilized with liquid fraction, inoculated with furfural preculture (dotted line), batch SSF solubilized with water, inoculated with furfural preculture (15% V/DW enzymes) (straight line), two-stage SSF solubilized with water, inoculated with furfural preculture (dashed line)

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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