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Table 1 Effects of vitamins on growth and TAG content of two diatoms, C. gracilis and P. tricornutum

From: Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer

  Inoculation at logarithmic phase Inoculation at stationary phase
+ vitamins − vitamins + vitamins − vitamins
C. gracilis
 Doubling time (h) 10.7 ± 0.6 12.0 ± 0.6 12.6 ± 0.6 12.5 ± 0.3
 Cell density on 4th day (106 cells/mL) 4.14 ± 0.22 3.26 ± 0.07 4.08 ± 0.15 3.30 ± 0.07
 Cell density on 10th day (106 cells/mL) 6.28 ± 0.25 6.68 ± 0.04 6.48 ± 0.27 6.08 ± 0.19
 Volume-specific TAG on 10th day (mg/L) 232 ± 9 190 ± 5 216 ± 9 209 ± 3
 Cell-specific TAG on 10th day (pg/cell) 38.5 ± 1.1 29.0 ± 0.2 33.3 ± 0.1 34.4 ± 0.8
P. tricornutum
 Doubling time (h) 14.5 ± 0.1 14.4 ± 0.2 17.5 ± 0.8 20.1 ± 0.9
 Cell density on 4th day (106 cells/mL) 15.3 ± 0.6 14.9 ± 0.2 14.1 ± 0.2 9.68 ± 0.52
 Cell density on 10th day (106 cells/mL) 17.5 ± 0.3 18.4 ± 0.3 15.8 ± 0.2 14.7 ± 0.6
 Volume-specific TAG on 10th day (mg/L) 179 ± 4 181 ± 5 152 ± 6 54.5 ± 5.4
 Cell-specific TAG on 10th day (pg/cell) 10.2 ± 0.3) 9.84 ± 0.20) 9.92 ± 0.67 3.69 ± 0.20
  1. Cells cultivated in F/2-enriched seawater with or without vitamins (B12, biotin, and thiamine-HCl), and n = 3–4