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Table 1 Effects of vitamins on growth and TAG content of two diatoms, C. gracilis and P. tricornutum

From: Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer

 

Inoculation at logarithmic phase

Inoculation at stationary phase

+ vitamins

− vitamins

+ vitamins

− vitamins

C. gracilis

 Doubling time (h)

10.7 ± 0.6

12.0 ± 0.6

12.6 ± 0.6

12.5 ± 0.3

 Cell density on 4th day (106 cells/mL)

4.14 ± 0.22

3.26 ± 0.07

4.08 ± 0.15

3.30 ± 0.07

 Cell density on 10th day (106 cells/mL)

6.28 ± 0.25

6.68 ± 0.04

6.48 ± 0.27

6.08 ± 0.19

 Volume-specific TAG on 10th day (mg/L)

232 ± 9

190 ± 5

216 ± 9

209 ± 3

 Cell-specific TAG on 10th day (pg/cell)

38.5 ± 1.1

29.0 ± 0.2

33.3 ± 0.1

34.4 ± 0.8

P. tricornutum

 Doubling time (h)

14.5 ± 0.1

14.4 ± 0.2

17.5 ± 0.8

20.1 ± 0.9

 Cell density on 4th day (106 cells/mL)

15.3 ± 0.6

14.9 ± 0.2

14.1 ± 0.2

9.68 ± 0.52

 Cell density on 10th day (106 cells/mL)

17.5 ± 0.3

18.4 ± 0.3

15.8 ± 0.2

14.7 ± 0.6

 Volume-specific TAG on 10th day (mg/L)

179 ± 4

181 ± 5

152 ± 6

54.5 ± 5.4

 Cell-specific TAG on 10th day (pg/cell)

10.2 ± 0.3)

9.84 ± 0.20)

9.92 ± 0.67

3.69 ± 0.20

  1. Cells cultivated in F/2-enriched seawater with or without vitamins (B12, biotin, and thiamine-HCl), and n = 3–4