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Table 5 Use of sewage water as the nutrient source in culture media

From: Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer

  50% sewage water 67% sewage water
1st subculture 2nd subculture 1st subculture 2nd subculture
Doubling time (h) 12.9 ± 0.4 19.2 ± 0.5 15.4 ± 0.5 18.3 ± 0.1
Cell density on 4th day (106 cells/mL) 5.06 ± 0.02 4.24 ± 0.04 5.29 ± 0.39 4.79 ± 0.05
Cell density on 10th day (106 cells/mL) 6.17 ± 0.08 4.95 ± 0.01 6.19 ± 0.14 5.99 ± 0.05
Volume-specific TAG on 10th day (mg/L) 156 ± 4 135 ± 9 203 ± 9 164 ± 7
Cell-specific TAG on 10th day (pg/cell) 25.2 ± 0.6 27.3 ± 1.8 32.8 ± 1.4 27.4 ± 1.0
  1. Chaetoceros gracilis cultured in seawater mixed with one or two volumes of sewage water (50 and 67% sewage water), cells used to inoculate sewage water media cultivated first in F/2-enriched seawater (1st subculture) or subcultured once in same sewage water medium (2nd subculture), and n = 3