Skip to main content

Table 5 Use of sewage water as the nutrient source in culture media

From: Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer

 

50% sewage water

67% sewage water

1st subculture

2nd subculture

1st subculture

2nd subculture

Doubling time (h)

12.9 ± 0.4

19.2 ± 0.5

15.4 ± 0.5

18.3 ± 0.1

Cell density on 4th day (106 cells/mL)

5.06 ± 0.02

4.24 ± 0.04

5.29 ± 0.39

4.79 ± 0.05

Cell density on 10th day (106 cells/mL)

6.17 ± 0.08

4.95 ± 0.01

6.19 ± 0.14

5.99 ± 0.05

Volume-specific TAG on 10th day (mg/L)

156 ± 4

135 ± 9

203 ± 9

164 ± 7

Cell-specific TAG on 10th day (pg/cell)

25.2 ± 0.6

27.3 ± 1.8

32.8 ± 1.4

27.4 ± 1.0

  1. Chaetoceros gracilis cultured in seawater mixed with one or two volumes of sewage water (50 and 67% sewage water), cells used to inoculate sewage water media cultivated first in F/2-enriched seawater (1st subculture) or subcultured once in same sewage water medium (2nd subculture), and n = 3