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Table 9 Pigment contents in Chaetoceros gracilis cells grown in various culture media

From: Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer

Pigments

Amount of pigments (mg/L)

F/2

Urea-F/2

67% sewage water

2% liquid fertilizer

96 h

240 h

96 h

240 h

96 h

240 h

96 h

240 h

Chlorophyll a

3.77 ± 0.22

3.22 ± 0.17

3.45 ± 0.16

3.00 ± 0.09

0.778 ± 0.106

0.710 ± 0.043

2.85 ± 0.24

2.33 ± 0.04

Chlorophyll c

0.778 ± 0.050

0.685 ± 0.040

0.672 ± 0.028

0.614 ± 0.014

0.163 ± 0.018

0.136 ± 0.010

0.549 ± 0.036

0.399 ± 0.006

Fucoxanthin

2.01 ± 0.02

1.99 ± 0.10

1.95 ± 0.16

1.89 ± 0.05

0.433 ± 0.063

0.444 ± 0.029

1.39 ± 0.01

1.22 ± 0.05

Diadinoxanthin

0.535 ± 0.059

0.564 ± 0.007

0.493 ± 0.020

0.529 ± 0.018

0.140 ± 0.019

0.193 ± 0.013

0.361 ± 0.071

0.375 ± 0.011

ß-Carotene

0.115 ± 0.004

0.0759 ± 0.0025

0.0681 ± 0.0310

0.0695 ± 0.0022

0.0237 ± 0.0045

0.0255 ± 0.0010

0.0841 ± 0.0033

0.0670 ± 0.0006

Phosphate (ppm)

3.46

3.46

1.3

1.73

Nitrate (ppm)

45

0

0

0

Ammonium (ppm)

0

0

6.7

33

Cell density on 4th day (106 cells/mL)

4.25 ± 0.37

4.90 ± 0.31

2.32 ± 0.49

4.02 ± 0.30

Cell density on 10th day (106 cells/mL)

6.19 ± 0.1

6.43 ± 0.47

4.05 ± 0.41

4.30 ± 0.22

  1. Chaetoceros gracilis cultured in F/2-enriched seawater inoculated in F/2-enriched seawater, Urea-F/2 medium contained urea not nitrate at same molar concentration, seawater mixed with two volumes of sewage water (67% sewage water medium) or seawater mixed with one-fifth of 10% (v/v) liquid fertilizer (2% liquid fertilizer medium), pigments quantitated on days 4 and 10, and n = 3