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Table 9 Pigment contents in Chaetoceros gracilis cells grown in various culture media

From: Advantageous characteristics of the diatom Chaetoceros gracilis as a sustainable biofuel producer

Pigments Amount of pigments (mg/L)
F/2 Urea-F/2 67% sewage water 2% liquid fertilizer
96 h 240 h 96 h 240 h 96 h 240 h 96 h 240 h
Chlorophyll a 3.77 ± 0.22 3.22 ± 0.17 3.45 ± 0.16 3.00 ± 0.09 0.778 ± 0.106 0.710 ± 0.043 2.85 ± 0.24 2.33 ± 0.04
Chlorophyll c 0.778 ± 0.050 0.685 ± 0.040 0.672 ± 0.028 0.614 ± 0.014 0.163 ± 0.018 0.136 ± 0.010 0.549 ± 0.036 0.399 ± 0.006
Fucoxanthin 2.01 ± 0.02 1.99 ± 0.10 1.95 ± 0.16 1.89 ± 0.05 0.433 ± 0.063 0.444 ± 0.029 1.39 ± 0.01 1.22 ± 0.05
Diadinoxanthin 0.535 ± 0.059 0.564 ± 0.007 0.493 ± 0.020 0.529 ± 0.018 0.140 ± 0.019 0.193 ± 0.013 0.361 ± 0.071 0.375 ± 0.011
ß-Carotene 0.115 ± 0.004 0.0759 ± 0.0025 0.0681 ± 0.0310 0.0695 ± 0.0022 0.0237 ± 0.0045 0.0255 ± 0.0010 0.0841 ± 0.0033 0.0670 ± 0.0006
Phosphate (ppm) 3.46 3.46 1.3 1.73
Nitrate (ppm) 45 0 0 0
Ammonium (ppm) 0 0 6.7 33
Cell density on 4th day (106 cells/mL) 4.25 ± 0.37 4.90 ± 0.31 2.32 ± 0.49 4.02 ± 0.30
Cell density on 10th day (106 cells/mL) 6.19 ± 0.1 6.43 ± 0.47 4.05 ± 0.41 4.30 ± 0.22
  1. Chaetoceros gracilis cultured in F/2-enriched seawater inoculated in F/2-enriched seawater, Urea-F/2 medium contained urea not nitrate at same molar concentration, seawater mixed with two volumes of sewage water (67% sewage water medium) or seawater mixed with one-fifth of 10% (v/v) liquid fertilizer (2% liquid fertilizer medium), pigments quantitated on days 4 and 10, and n = 3