Effective isopropanol–butanol (IB) fermentation with high butanol content using a newly isolated Clostridium sp. A1424

Table 2 Fermentation products of Clostridium sp. A1424 using various substrates

Substrate	Substrate consumed (g/L)	Products (g/L)						Yield (g/g)
Substrate	Substrate consumed (g/L)	Butanol	Isopropanol	Acetone	Acetic acid	Butyric acid	1,3-propanediol	Y ^a_B	Y ^a_IB
Glucose	21.62_±0.06	4.49_±0.04	2.48_±0.06	0.33_±0.03	0.43_±0.06	0.72_±0.02	0	0.21	0.32
Mannose	19.92_±0.04	4.40_±0.13	1.88_±0.01	0.33_±0.04	0.66_±0.02	0.93_±0.05	0	0.24	0.34
Fructose	21.63_±0.05	4.38_±0.02	1.63_±0.06	0.19_±0.01	0.85_±0.01	1.49_±0.03	0	0.23	0.31
Galactose	5.96_±0.14	0.59_±0.01	0	0	0.44_±0.02	1.90_±0.02	0	0.11	0.11
Xylose	12.81_±0.09	0.87_±0.03	0	0	0.68_±0.02	4.71_±0.00	0	0.08	0.08
Arabinose	8.24_±0.04	0	0	0	0.15_±0.01	4.20_±0.05	0	0	0
Cellobiose	20.02_±0.04	4.50_±0.02	2.65_±0.00	0.45_±0.01	0.43_±0.01	0.24_±0.01	0	0.23	0.37
Sucrose	18.20_±1.13	5.01_±0.04	1.71_±0.02	0.34_±0.02	0.97_±0.00	0.87_±0.07	0	0.28	0.37
Glycerol	18.13_±1.10	3.91_±0.19	0	0	0.27_±0.02	2.20_±0.07	3.40_±0.25	0.23	0.23

The initial substrate concentration was 18–22 g/L for each substrate
The fermentation was performed on serum bottle without pH control
The data were obtained at 28 h of fermentation except sucrose cultures (36 h)
The values are average and one standard deviation of triplicate experiments
^a Y _B, butanol yield (g butanol/g substrate); Y _IB, the sum of butanol and isopropanol yield (g [butanol + isopropanol]/g substrate)

ISSN: 2731-3654