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Table 2 Hydrolysis of delignified corn stover by commercial and EMSD5 enzyme preparations

From: Metagenomic and metaproteomic analyses of a corn stover-adapted microbial consortium EMSD5 reveal its taxonomic and enzymatic basis for degrading lignocellulose

Enzyme addition Enzyme mixture Protein loading
(mg/g substrate)
Cellulose conversion (%) Xylan conversion (%) Degree of synergism
Individual 10 mg C 10 27.1 ± 0.6 2.2 ± 0.3 n.a.
9 mg C 9 25.5 ± 0.3 2.1 ± 0.1 n.a.
7.5 mg C 7.5 20.9 ± 0.3 1.9 ± 0.1 n.a.
5 mg C 5 14.7 ± 0.2 1.4 ± 0.2 n.a.
2.5 mg C 2.5 12.1 ± 0.4 1.0 ± 0.1 n.a.
10 mg E 10 8.1 ± 0.2 18.7 ± 0.4 n.a.
7.5 mg E 7.5 7.4 ± 0.3 16.3 ± 0.2 n.a.
5 mg E 5 6.3 ± 0.1 12.2 ± 0.5 n.a.
2.5 mg E 2.5 2.3 ± 0.1 5.6 ± 0.2 n.a.
1 mg E 1 1.2 ± 0.3 2.7 ± 0.2 n.a.
Supplement 10 mg C + 2.5 mg E 12.5 34.7 ± 0.7 9.1 ± 0.5 1.18
10 mg C + 5 mg E 15 40.5 ± 1.8 16.0 ± 0.5 1.21
10 mg C + 7.5 mg E 17.5 43.1 ± 1.4 20.5 ± 0.8 1.25
10 mg C + 10 mg E 20 44.6 ± 2.3 23.4 ± 0.6 1.27
Replace 9 mg C + 1 mg E 10 30.6 ± 0.9 4.9 ± 0.5 1.14
7.5 mg C + 2.5 mg E 10 35.8 ± 0.6 8.8 ± 0.3 1.54
5 mg C + 5 mg E 10 36.3 ± 1.7 15.1 ± 1.0 1.73
2.5 mg C + 7.5 mg E 10 38.3 ± 1.3 18.7 ± 1.1 1.96
  1. Hydrolysis assays were performed at 50 °C for 24 h at 2% (w/v) substrate loading
  2. The mean values of three replicates and standard deviations are presented
  3. C commercial enzyme preparation, E EMSD5 enzyme preparation. n.a. not applicable