Table 1 Bacterial strains, plasmids, and primers used in this study

Bacterial strains

 Leuconostoc mesenteroides

KCTC 3718 wild type strain carrying pMBLT03

This study

  LMS50

Adapted mutant strain in MRS medium supplemented with 50 g/L lactic acid

This study

  LMS60

Adapted mutant strain in MRS medium supplemented with 60 g/L lactic acid

This study

  LMS70

Adapted mutant strain in MRS medium supplemented with 70 g/L lactic acid

This study

 Escherichia coli

  XL1-blue

Cloning host for pSTV28

Stratagene

Plasmids

 pMBLT03

E. coli-Leuconostoc shuttle vector containing ldhD gene from Lactobacillus plantarum; Em^R

 pSTV28

P15A derived cloning vector; Cm^R

TAKARA

 pSTV_atpC_WT

Expressed atpC of Ln mesenteroides in E. coli; Cm^R

This study

 pSTV_atpC_MU

Expressed atpC of LMA70 in E. coli; Cm^R

This study

Primers

 atpC_WT_F

5′-GGAATTCAGGAGGATTACAAAATGGCAGATGAAACAACAAC-3′

 atpC_WT_R

5′-CCCAAGCTTATTTAGCGACCAGATTTCAG-3′

 atpC_Mutant_F

5′-GGAATTCAGGAGGATTACAAAATGGCAGATGAAACAACAAC-3′

 atpC_Mutant_R

5′-CCCAAGCTTTTTATCGGCTACAAACTGTCAA-3′