Long-term adaptive evolution of Leuconostoc mesenteroides for enhancement of lactic acid tolerance and production

Table 1 Bacterial strains, plasmids, and primers used in this study

Strains or plasmids	Relevant properties	Source or reference
Bacterial strains
Leuconostoc mesenteroides	KCTC 3718 wild type strain carrying pMBLT03	This study
LMS50	Adapted mutant strain in MRS medium supplemented with 50 g/L lactic acid	This study
LMS60	Adapted mutant strain in MRS medium supplemented with 60 g/L lactic acid	This study
LMS70	Adapted mutant strain in MRS medium supplemented with 70 g/L lactic acid	This study
Escherichia coli
XL1-blue	Cloning host for pSTV28	Stratagene
Plasmids
pMBLT03	E. coli-Leuconostoc shuttle vector containing ldhD gene from Lactobacillus plantarum; Em^R	[9]
pSTV28	P15A derived cloning vector; Cm^R	TAKARA
pSTV_atpC_WT	Expressed atpC of Ln mesenteroides in E. coli; Cm^R	This study
pSTV_atpC_MU	Expressed atpC of LMA70 in E. coli; Cm^R	This study
Primers
atpC_WT_F	5′-GGAATTCAGGAGGATTACAAAATGGCAGATGAAACAACAAC-3′
atpC_WT_R	5′-CCCAAGCTTATTTAGCGACCAGATTTCAG-3′
atpC_Mutant_F	5′-GGAATTCAGGAGGATTACAAAATGGCAGATGAAACAACAAC-3′
atpC_Mutant_R	5′-CCCAAGCTTTTTATCGGCTACAAACTGTCAA-3′

ISSN: 2731-3654