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Table 1 Bacterial strains, plasmids, and primers used in this study

From: Long-term adaptive evolution of Leuconostoc mesenteroides for enhancement of lactic acid tolerance and production

Strains or plasmids Relevant properties Source or reference
Bacterial strains
 Leuconostoc mesenteroides KCTC 3718 wild type strain carrying pMBLT03 This study
  LMS50 Adapted mutant strain in MRS medium supplemented with 50 g/L lactic acid This study
  LMS60 Adapted mutant strain in MRS medium supplemented with 60 g/L lactic acid This study
  LMS70 Adapted mutant strain in MRS medium supplemented with 70 g/L lactic acid This study
 Escherichia coli
  XL1-blue Cloning host for pSTV28 Stratagene
Plasmids
 pMBLT03 E. coli-Leuconostoc shuttle vector containing ldhD gene from Lactobacillus plantarum; EmR [9]
 pSTV28 P15A derived cloning vector; CmR TAKARA
 pSTV_atpC_WT Expressed atpC of Ln mesenteroides in E. coli; CmR This study
 pSTV_atpC_MU Expressed atpC of LMA70 in E. coli; CmR This study
Primers
 atpC_WT_F 5′-GGAATTCAGGAGGATTACAAAATGGCAGATGAAACAACAAC-3′  
 atpC_WT_R 5′-CCCAAGCTTATTTAGCGACCAGATTTCAG-3′  
 atpC_Mutant_F 5′-GGAATTCAGGAGGATTACAAAATGGCAGATGAAACAACAAC-3′  
 atpC_Mutant_R 5′-CCCAAGCTTTTTATCGGCTACAAACTGTCAA-3′