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Table 2 Steady-state kinetic parameters for the oxidation of VE dimer for wild-type and mutants

From: Improvement of catalytic performance of lignin peroxidase for the enhanced degradation of lignocellulose biomass based on the imbedded electron-relay in long-range electron transfer route

Variants Oxidation of VE dimer
KM (mM) kcat (s−1) kcat/KM (s−1 mM−1)
WT 0.13 ± 0.03 0.77 ± 0.05 5.59 ± 0.69
W251A 0.26 ± 0.01 0.06 ± 0.01 0.25 ± 0.04
W251F 0.15 ± 0.01 0.61 ± 0.08 4.10 ± 0.52
W251Y 0.16 ± 0.01 0.45 ± 0.01 2.81 ± 0.05
T208D 0.38 ± 0.01 2.44 ± 0.08 6.40 ± 0.11
A242D 0.55 ± 0.02 16.48 ± 0.20 29.96 ± 0.92
T208D/A242D 1.22 ± 0.03 16.13 ± 0.35 13.22 ± 0.31