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Table 2 Steady-state kinetic parameters for the oxidation of VE dimer for wild-type and mutants

From: Improvement of catalytic performance of lignin peroxidase for the enhanced degradation of lignocellulose biomass based on the imbedded electron-relay in long-range electron transfer route

Variants

Oxidation of VE dimer

KM (mM)

kcat (s−1)

kcat/KM (s−1 mM−1)

WT

0.13 ± 0.03

0.77 ± 0.05

5.59 ± 0.69

W251A

0.26 ± 0.01

0.06 ± 0.01

0.25 ± 0.04

W251F

0.15 ± 0.01

0.61 ± 0.08

4.10 ± 0.52

W251Y

0.16 ± 0.01

0.45 ± 0.01

2.81 ± 0.05

T208D

0.38 ± 0.01

2.44 ± 0.08

6.40 ± 0.11

A242D

0.55 ± 0.02

16.48 ± 0.20

29.96 ± 0.92

T208D/A242D

1.22 ± 0.03

16.13 ± 0.35

13.22 ± 0.31

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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