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Table 2 The macromolecular composition of T. reesei

From: Whole-genome metabolic model of Trichoderma reesei built by comparative reconstruction

Biomass component

% (w/w)

Proteins

45.100 a

 Ala

2.715b

 Arg

3.944b

 Asn

1.613b

 Asp

2.677b

 Cys

0.549b

 Gln

2.063b

 Glu

3.200b

 Gly

1.649b

 His

1.346b

 Ile

2.186b

 Leu

4.101b

 Lys

2.595b

 Met

1.202b

 Phe

2.220b

 Pro

2.363b

 Ser

2.808b

 Thr

2.354b

 Trp

1.144b

 Tyr

1.846b

 Val

2.523b

Carbohydrates

23.190 a

 Chitin

7.820c

 Other carbohydrates

15.370d

RNA

6.122 a b

DNA

0.912 a

Lipids

4.176 g

 Fatty acids-esters

  Myristic acid (C14:0) est

0.004a

  Palmitic acid (C16:0) est

0.613a

  Palmitoleic acid (C16:1n-7) est

0.011a

  Stearic acid (C18:0) est

0.070a

  Oleic acid (C18:1n-9) est

0.126a

  Linoleic acid (C18:2n-6) est

1.425a

  \(\alpha\)-linolenic acid (C18:3n-3) est

0.292a

  Arachidic acid (C20:0) est

0.004a

  Lignoceric acid (C24:0) est

0.005a

 Fatty acids-free

  Palmitic acid (C16:0) FFA

0.060a

  Stearic acid (C18:0) FFA

0.012a

  Oleic acid (C18:1n-9) FFA

0.107a

  Linoleic acid (C18:2n-6) FFA

0.274a

 Ergosterol

0.278a

 Triacylglycerol

1.792e

 Phosphatidylethanolamine

0.551e

 Phosphatidylcholine

1.102e

Ash

5.100 f

  1. aMeasured as described in "Methods" section
  2. b Amino acid ratios calculated based on codon ratios in RNAseq transcriptome data, with transcripts encoding secreted proteins removed
  3. c Estimated based on the chitin content (% w/w) of A. oryzae biomass [8], and corrected for different ash contents in A. oryzae and T. reesei preparates
  4. d Carbohydrates other than chitin
  5. e Calculated based on the measured ratio of TAG:PE:PC 52:16:32 and the measured amount of fatty acid esters
  6. f Measured from lactose-limited chemostat cultures of T. reesei Rut-C30 (D = 0.051/h) described in [40]
  7. g Sum of measured free fatty acids, ergosterol, triacylglycerol, phosphatidylethanolamine and phosphatidylcholine