Fig. 2From: Increasing n-butanol production with Saccharomyces cerevisiae by optimizing acetyl-CoA synthesis, NADH levels and trans-2-enoyl-CoA reductase expressionSpotting assay of adhEA267T/E568K/R577S strains. Cells were grown to exponential phase and adjusted to an OD600 of 1. Tenfold serial dilutions were spotted onto YEPD or YEPE under aerobic conditions and incubated at 30 °C for 3 days. As a control, wild-type BY4741 (Y00000) was usedBack to article page