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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Increasing n-butanol production with Saccharomyces cerevisiae by optimizing acetyl-CoA synthesis, NADH levels and trans-2-enoyl-CoA reductase expression

Fig. 3

Spotting assay of pADH1_FMS1 strains on SCD without pantothenate, containing Δfms1 cells. Cells were grown to exponential phase and adjusted to an OD600 of 1. Tenfold serial dilutions were spotted onto SCD lacking pantothenate, whereby 10 µl of Δfms1 overnight culture was added to the medium. Control VSY13 (Δadh16 sfa1 gpd2, n-butanol pathway genes, coaA and adhE A267T/E568K/R577S) and VSY15 (VSY13 with pADH1_FMS1) were compared. Cells were incubated aerobically at 30 °C for 4 days

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