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Fig. 4 | Biotechnology for Biofuels

Fig. 4

From: Increasing n-butanol production with Saccharomyces cerevisiae by optimizing acetyl-CoA synthesis, NADH levels and trans-2-enoyl-CoA reductase expression

Fig. 4

n-Butanol concentrations of n-butanol-producing yeast strains additionally overexpressing enzymes of the n-butanol biosynthesis pathway. Shown are maximum n-butanol titers (Ca hbd and Td ter after 74 h and others after 100 h) of aerobic fermentations in SMD media. Bottleneck analysis was carried out with VSY15 (Δadh1-6 sfa1 gpd2 with n-butanol pathway genes, coaA and adhE A267T/E568K/R577S, pADH1_FMS1) overexpressing pRS62H (high copy vector, promoter pHXT7, terminator tFBA1) with one each enzyme of n-butanol pathway. As a control, empty vector pRS62H was used. Genes from Sc: Saccharomyces cerevisiae, Ca: Clostridium acetobutylicum, Ec: E.coli, and Td: Treponema denticola are indicated by prefixes in superscript. Error bars represent the standard deviation of three independent replicates

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